Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.13/520
Título: Enzymatic inhibitory activity of hydroxycinnamates (HCs): in silico studies
Autor: Serina, José João Caires
Orientador: Castilho, Paula Cristina Machado Ferreira
Fernandes, Miguel Xavier Jesus Josefat
Palavras-chave: Diabetes
α-glucosidase
Caffeoyl quinic acid
Molecular docking
Inhibitor
In situ drug design
Bioquímica Aplicada
.
Centro de Ciências Exatas e da Engenharia
Data de Defesa: 13-Jan-2014
Resumo: Diabetes is a worldwide health issue that has been expanding mainly in developed countries. It is characterized by abnormal levels of blood sugar due to several factors. The most common are resistance to insulin and the production of defective insulin which exerts little or no effect. Its most common symptoms include tissue damage to several systems due to elevated levels of blood sugar. One of the key enzymes in hydrocarbon metabolism is α-glucosidase (EC 3.2.1.20). It catalyzes the breakdown of complex carbohydrates into their respective monomers (glucose) which allows them to be absorbed. In this work, caffeoyl quinic acids and their metabolites were analyzed as potential inhibitors for α-glucosidase. The search for the best inhibitor was conducted using molecular docking. The affinity of each compound was compared to the inhibitor present in the crystal structure of the protein. As no inhibitor with a similar affinity was´found, a new approach was used, in situ drug design. It was not possible to achieve an inhibitor capable of competing with the one present in the crystal structure of the enzyme, which is also its current commercial inhibitor. It is possible to draw some conclusions as to which functional groups interact best with certain residues of the active site. This work was divided into three main sections. The first section, Diabetes, serves as an introduction to what is Diabetes, its symptoms and/or side effects and how caffeoyl quinic acids could be used as a treatment. The second section, Caffeoylquinic acids and their metabolites as inhibitors for Alfa-glucosidase, corresponds to the search through molecular docking of caffeoyl quinic acids as inhibitors for α-glucosidase and what was possible to draw from this search. The last section, In situ design of an inhibitor for α-glucosidase (EC 3.2.1.20), corresponds to the in situ drug design study and what it achieved. The representation of each of the molecules used as a ligand can be found in the Annexes.
URI: http://hdl.handle.net/10400.13/520
Designação: Master in Applied Biochemistry
Aparece nas colecções:Dissertações de Mestrado

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