Browsing by Author "Pinto, F. M."
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- Population genetic structure and colonization sequence of Drosophila subobscura in the Canaries and Madeira Atlantic islands as inferred by autosomal, sex-linked and mtDNA traitsPublication . Pinto, F. M.; Brehm, A.; Hernández, M.; Larruga, J. M.; González, A. M.; Cabrera, V. M.The genetic structure In Atlantic Islands and continental populations of Drosophila subobscura has been studied using autosomal and sex-linked allozymes and ml tochondrial DNA (mtDNA) haplotypes. From the data it Is deduced that whereas the Canary Islands have long been isolated, the neighboring island of Madeira has been subjected to continuous migration from the mainland. In addition, sex-linked allo zymes and mtDNA data show a large divergence between the geologically younger western Islands of the Canarian Archipelago and the older central ones, finding strong founder effects In the former. Divergence rates of sex-linked and mltochon drlal genes relative to autosomlc loci several times higher than expected under neutrality have been explained by differential migration between sexes. The Ca narian Archipelago colonization fits in well with a stepping-stone model of a direc tional east-west migration that parallels the geological origin of these Islands.
- Structure and evolution of the mitochondrial DNA complete control region in the Drosophila subobscura subgroupPublication . Brehm, A.; Harris, D. J.; Hernández, M.; Cabrera, V. M.; Larruga, J. M.; Pinto, F. M.; González, A. M.The complete A + T-rich region of mitochondrial DNA (mtDNA) has been cloned and sequenced in the species of the Drosophila subobscura subgroup D. subobscura, D. madeirensis and D. guanche. Comparative analysis of these sequences with others already published has identified new sequence motifs that are conserved in Drosophila and other insects. A putative bi-directional promoter and a stop signal are proposed to be involved in the primary mtDNA strand replication of Drosophila. This region strongly resolves relationships of the species included in a phylogenetic analysis, both for closely related species and also at deeper phylogenetic levels when only the left and central domains are taken into account.