Nesic, Maja D.Ducic, TanjaGonçalves, MaraStepić, MilutinAlgarra, ManuelSoto, JuanGemović, BranislavaBandosz, Teresa J.Petkovic, Marijana2023-06-192023-06-192022Nešić, M. D., Dučić, T., Gonçalves, M., Stepić, M., Algarra, M., Soto, J., ... & Petković, M. (2022). Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. Chemico-Biological Interactions, 360, 109950.http://hdl.handle.net/10400.13/5234Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respec tively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination.engAnticancer photodynamic therapyBiochemical changesN-doped carbon dotsCarbon dotsRu-metallodrug.Faculdade de Ciências Exatas e da Engenhariajournal article10.1016/j.cbi.2022.109950