Nogueira, NatachaCordeiro, NereidaCanada, PaulaSilva, Paula Cruz eOzório, Rodrigo O. A.2021-05-042021-05-042010Nogueira, N., Cordeiro, N., Canada, P., Cruz e Silva, P., & Ozório, R. O. (2010). Separate and combined effects of cyclic fasting and l‐carnitine supplementation in red porgy (Pagrus pagrus, L. 1758). Aquaculture Research, 41(11), e795-e806.http://hdl.handle.net/10400.13/3357We examined the e¡ects of cyclic fasting in red porgy (Pagrus pagrus) fed di¡erent dietary carnitine levels. Ju venile ¢sh (23.58 3.49 g) were divided into eight groups ^ four groups were fed every day to apparent sa tiation, while the other four were fasted for 7 days every 2 weeks. In each feeding regime, two replicates were fed an L-carnitine non-supplemented diet (46 mg kg 1 ) and the other two groups were fed an L-carnitine sup plemented diet (630 mg kg 1 ). Fish fed 630 mg L-carni tine accumulated two times more L-carnitine in muscle than ¢sh fed 46 mg L-carnitine. Cyclic fasting reduced the growth performance and lipid content in the liver. Carnitine supplementation did not a¡ect performance and body composition, but decreased the n-6 PUFA content. Moreover, the combined e¡ects of fasting and carnitine supplementation were observed on reducing the n-3 fatty acid content. Areas of steatosis were found in the livers of red porgy, but the results revealed that supplementation of L-carnitine in cyclic fasted ¢sh con tributed towards a lower degree of vacuolization than in ¢sh fed to apparent satiation. Regardless of the feed ing regime applied, the spleen of ¢sh fed the L-carni tine-supplemented diet was haemorrhagic and hyper activation of melanomacrophage cells was observed.engFastingCompensatory growthCarnitineRed porgy.Faculdade de Ciências Exatas e da Engenhariajournal article10.1111/j.1365-2109.2010.02596.x