Ragonezi, CarlaZavattieri, Maria2022-02-042022-02-042018Ragonezi, C., & Zavattieri, M. A. (2018). Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots. Methods and Protocols, 1(3), 34. https://doi.org/10.3390/mps1030034http://hdl.handle.net/10400.13/4046Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.engEctomycorrhizaMycorrhiza-like structuresStone pineAdventitious rootsHartig net.Escola Superior de Tecnologias e Gestãojournal article10.3390/mps1030034