Castro, Mário Rui da Costa Basílio eRagonezi, CarlaOliveira, Paulo Guilherme Leandro deZavattieri, Maria Amely2022-02-042022-02-042019Castro, M., Ragonezi, C., de Oliveira, P., & Zavattieri, M. (2019). A novelty system for biotization of plant microshoots and collection of natural compounds. Methods and Protocols, 2(1). http://dx.doi.org/10.3390/mps2010005http://hdl.handle.net/10400.13/4042An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.engBiotechnologyBiotizationChemical analysisDouble-phase mediumPhenolic compoundsLiquid chromatographyDiode array detectorMass spectrometry.Escola Superior de Tecnologias e Gestãojournal article10.3390/mps2010005