Spínola, HélderBruges-Armas, JácomeBrehm, António2017-01-262017-01-262007Spínola, H., Bruges-Armas, J., & Brehm, A. (2007). Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: A Case Study. Human Biology, 79 (5), 537-543.0018-7143http://hdl.handle.net/10400.13/1351Six hundred twenty-one samples from Portugal, the Cabo Verde archipelago, and Guinea-Bissau were typed for HLA-A, HLA-B, and HLADRB1usingthepolymerasechainreaction–sequence-specificoligonucleotide probe (PCR-SSOP) method and the sequence-based typing (SBT) method to characterizeandcomparediscrepanciesbetweenthetwomethods.Fifty-three alleles (4.27% of 1,242 chromosomes typed) identified by the PCR-SSOP method were not concordant with the results obtained using the SBT method. Thirty-four (2.74% of total chromosomes typed) PCR-SSOP mistyping results were discrepancies inside the same allele group and 19 others (1.53% of total chromosomes typed) were relative to nonconcordant results between different groups. PCR-SSOP allele mistyping is the result of interpretation difficulties resulting from less intense, absent, or dubious hybridization patterns. Noncommercial PCR-SSOP procedures are highly exigent on the technicians’ experience and the availability of properly calibrated high-precision equipment.engHLAPolymerase chain reaction - sequence-specific - oligonucleotide probe (PCR-SSOP) techniqueSequence-based typing (SBT) techniquePortugalCabo VerdeGuiné-BissauAzores islands (Portugal).journal article