Zhu, BeitongWu, XiaoliRodrigues, JoãoHu, XichaoSheng, RuilongBao, Guang-Ming2022-02-172022-02-172021Zhu, B., Wu, X., Rodrigues, J., Hu, X., Sheng, R., & Bao, G. M. (2021). A dual-analytes responsive fluorescent probe for discriminative detection of ClO− and N2H4 in living cells. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 246, 118953. https://doi.org/10.1016/j.saa.2020.118953http://hdl.handle.net/10400.13/4088Hydrazine (N2H4) and ClO− are very harmful for public health, hence it is important and necessary to monitor them in living cells. Herein, we rationally designed and synthesized a dual-analytes responsive fluorescent sensor PTMQ for distinguishing detection of N2H4 and ClO−. PTMQ underwent N2H4-induced double bond cleavage, affording colorimetric and green fluorescence enhancement with good selectivity and a low detection limit (89 nM). On the other hand, PTMQ underwent ClO−-induced sulfur oxidation and displayed red fluorescence lighting-up response towards ClO− with good selectivity, rapid response (<0.2 min) and a low detection limit (58 nM). Moreover, PTMQ was successfully employed for in-situ imaging of N2H4 and ClO− in living cellsengFluorescent probeDual-analytesN2H4ClO−BioimagingLiving cells.Faculdade de Ciências Exatas e da EngenhariaCentro de Química da Madeirajournal article10.1016/j.saa.2020.118953