Fernandes, Laura SilvaCosta, Ygor Ferreira Garcia daBessa, Martha Eunice deFerreira, Adriana Lucia PiresCorrêa, José Otávio do AmaralDel-Vechio Vieira, GlauciemarSousa, Orlando Vieira deAraújo, Ana Lúcia Santos de MatosCastilho, Paula C.Alves, Maria Silvana2022-09-302022-09-302021Fernandes LS, da Costa YFG, de Bessa ME, Ferreira ALP, do Amaral Corrêa JO, et al. Metabolic profi ling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections. Arch Pharm Pharma Sci. 2021; 5: 020-028. DOI: 10.29328/journal.apps.1001027http://hdl.handle.net/10400.13/4676Morbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fi ght for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profi le and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profi le and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identifi ed. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and fi ve methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most eff ective (MIC ≤ 5,000 μg/μL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains.engEryngium pristisApiaceaeAnti bacterial activityBacterial resistanceStaphy lococcus aureusMethicillin-resistant Staphy lococcus aureus.Faculdade de Ciências Exatas e da EngenhariaCentro de Química da Madeirajournal article10.29328/journal.apps.1001027