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Publication
Study of drought tolerance responses in root and tuber crops using NIRS technology and biochemical markers model assessment
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Authors
Abstract(s)
Batata-doce (Ipomoea batatas L.) e inhame (Colocasia esculenta L.) representam a segunda fonte
alimentar mais importante do mundo. Atualmente, as alterações climáticas ameaçam a produtividade agrícola,
principalmente devido à escassez de água, cujo efeito não é ainda suficientemente compreendido. As principais
lacunas são a perceção do efeito da seca, através da eficiência no uso da água (WUE), bases bioquímicas e
fisiológicas de produtividade e qualidade. Aqui, apresentamos os modelos das respostas de sete acessos de
inhame e oito de batata-doce às condições de insuficiência hídrica, seguindo-se a avaliação da tolerância à
seca, através da criação de modelos de previsão rápidos e precisos baseados na espectroscopia próxima do
infravermelho (NIRS). Os resultados forneceram informações relevantes sobre o desenvolvimento destas
culturas em ambiente de stress. A escassez de água conduziu a mecanismos de redução da perda de água, com
diminuição da biomassa total (TPB) em todos os acessos. Respostas distintas na flexibilidade fenotípica foram
explicadas pela razão parte tuberosa/parte vegetativa e variação do índice de stress no crescimento dos órgãos.
Acessos com resposta mais favorável à seca melhoraram a WUE, com menor redução de TPB. Os parâmetros
de composição isotópica de carbono (δ13C) e azoto (δ15N) atuaram como integradores fisiológicos de resposta
ao stress ambiental. Maior teor de δ13C representou maior fixação de carbono, e maior variação de δ15N indicou
realocação generalizada de azoto entre os órgãos sob stress. O teor de oxalatos relacionou-se
significativamente com o amido, índice de clorofila e proteína. O desenvolvimento da técnica NIRS
demonstrou ser uma excelente ferramenta para detetar as respostas destas culturas à seca, através de parâmetros
de qualidade e fisiológicos, que poderá ser aplicada na investigação, pelos agricultores e empresas. Esta
informação também auxiliará na seleção de características a serem utilizadas nos programas de melhoramento
de inhame e batata-doce, para adaptação às mudanças climáticas.
Sweet potato (Ipomoea batatas L.) and taro (Colocasia esculenta L.) represent the world’s second most important food crop, as crucial carbohydrate source and good substitute for local imported cereals. Its productivity is currently threatened by climatic changes, mainly through the scarce of water availability, and affects tuber crops in a way that’s insufficiently understood. Major gaps are understanding how drought affects the sweet potato and taro whole-plants, throughout their water use efficiency (WUE), biochemical and physiological basis of productivity and quality. Here, we show seven taro and eight sweet potato accessions responses to water insufficiency conditions, following with the accurate models’ creation for drought tolerance assessment, through the rapid and precise prediction models based on Near-Infrared Spectroscopy (NIRS). Therefore, the results have provided new insights and relevant information about the taro and sweet potato whole-plant development under scarcity environment. The water shortage led to a drought avoidance response, with total plant biomass (TPB) loss in all accessions. Distinct phenotypic flexibility was also explained by the root-to-shoot ratio and stress index variation of the organs’ growth. Accessions with more favorable response to drought had improved WUE, with higher TPB. The carbon (δ13C) and nitrogen (δ15N) isotopic composition traits acted as physiological integrators of response to environmental stress. The ones with heaviest δ13C-values shown a greater carbon fixation, and δ15N variation indicating a generalized nitrogen reallocation between whole-plant organs under drought. The oxalate content was significantly related to starch content, chlorophyll content index, and protein. NIRS demonstrated to be an excellent tool to detect the crops drought responses, through physiological and quality traits, that could be applied for research purposes, or by farmers and agriculture companies. The information generated could also aid the selection of traits to be used in taro and sweet potato breeding programs, to adapt them to climate change.
Sweet potato (Ipomoea batatas L.) and taro (Colocasia esculenta L.) represent the world’s second most important food crop, as crucial carbohydrate source and good substitute for local imported cereals. Its productivity is currently threatened by climatic changes, mainly through the scarce of water availability, and affects tuber crops in a way that’s insufficiently understood. Major gaps are understanding how drought affects the sweet potato and taro whole-plants, throughout their water use efficiency (WUE), biochemical and physiological basis of productivity and quality. Here, we show seven taro and eight sweet potato accessions responses to water insufficiency conditions, following with the accurate models’ creation for drought tolerance assessment, through the rapid and precise prediction models based on Near-Infrared Spectroscopy (NIRS). Therefore, the results have provided new insights and relevant information about the taro and sweet potato whole-plant development under scarcity environment. The water shortage led to a drought avoidance response, with total plant biomass (TPB) loss in all accessions. Distinct phenotypic flexibility was also explained by the root-to-shoot ratio and stress index variation of the organs’ growth. Accessions with more favorable response to drought had improved WUE, with higher TPB. The carbon (δ13C) and nitrogen (δ15N) isotopic composition traits acted as physiological integrators of response to environmental stress. The ones with heaviest δ13C-values shown a greater carbon fixation, and δ15N variation indicating a generalized nitrogen reallocation between whole-plant organs under drought. The oxalate content was significantly related to starch content, chlorophyll content index, and protein. NIRS demonstrated to be an excellent tool to detect the crops drought responses, through physiological and quality traits, that could be applied for research purposes, or by farmers and agriculture companies. The information generated could also aid the selection of traits to be used in taro and sweet potato breeding programs, to adapt them to climate change.
Description
Keywords
Ácido abscísico Batata-doce (Ipomoea batatas (L.) Lam.) Espectroscopia próxima do infravermelho Inhame (Colocasia esculenta (L.) Schott) Integradores fisiológicos de seca Qualidade das culturas sob seca Abscisic acid Drought physiological integrators Near-infrared spectroscopy Quality of droughtstressed crops Sweet potato (Ipomoea batatas (L.) Lam.) Taro (Colocasia esculenta (L.) Schott) Biological Sciences . Faculdade de Ciências da Vida