Repository logo
 
Publication

Validation of a spectrophotometric methodology for a rapid iodine analysis in algae and seaweed casts

2019Journal article Open access
Simple item page
dc.contributor.authorNunes, N.
dc.contributor.authorValente, S.
dc.contributor.authorFerraz, S.
dc.contributor.authorBarreto, Maria Carmo
dc.contributor.authorCarvalho, M.A.A. Pinheiro de
dc.date.accessioned2021-03-08T15:00:43Z
dc.date.available2021-03-08T15:00:43Z
dc.date.issued2019
dc.description.abstractIodine plays an important role in human metabolism and its deficiency is particularly harmful in pregnancy and childhood. It remains a major public health concern in many countries, especially in Portugal. The main purpose of this work was to develop a validated spectrophotometric analysis for a fast and reliable iodine quantification in algal samples. Absorbance was determined at 410 nm demonstrating a good linearity (R2 ≈ 1.0) in the range of 0–0.06 mg I/100 g. LOD and LOQ were 1.7 × 10−3 and 5.0 × 10−3 mg I/100 g, respectively. Accuracy was determined using recovery and varied between 101 and 118%. For precision analysis, an intra-day test performance (RSD = 8.7%) and a repeatability assay (RSD = 3.8%) were performed. Matrix effect assessment demonstrated that this had a negligible effect (3.2%) in the iodine quantification. The spectrophotometric method was externally validated, for iodine quantification in algal samples, by INSA certified laboratory. The correlation coefficient between external iodine quantification and our work was R2 ≈ 0.9, showing a good correlation. Applicability was assessed in 25 macroalgae species (5 green, 9 red and 11 brown), 12 seaweed casts, collected in Canary Islands and 1 microalga (Isochrysis galbana) provided by ITC (Instituto Tecnologico de Canarias).pt_PT
dc.description.sponsorshipThis work was financially supported by ISOPlexis, University of Madeira; DemoBlueAlgae “Desenvolvimento de metodologias e optimização dos processos de cultivo e processamento de macroalgas para a indústria e economia azul” PROCiência 2020 (M1420-01-0247- FEDER000002); MACBIOBLUE “Proyecto demostrativo y de transferencia tecnológica para ayudar a las empresas a desarrollar nuevos produtos y procesos en el ambito de la Biotecnología Azul de la Macaronesia” (MAC/1.1b/086), program Interreg MAC 2014–2020; ARDITI - Regional Agency for the Development of Research Technology and Innovation (M14-20-09-5369-FSE-000001) Doctorate in Business and UBQ II, Unidade de Bioquímica (UBQ II company). Also, the authors present their acknowledgment to DRCT (Azores Regional Government) for co-financing, the Portuguese National Funds, through FCT-Fundação para a Ciência e a Tecnologia, and as applicable co-financed by the FEDER within the PT2020 Partnership Agreement by funding the cE3c centre (UID/BIA/00329/2013) and to DRCT for funding Azorean Biodiversity Group.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNunes, N., Valente, S., Ferraz, S., Barreto, M. C., & Carvalho, M. P. (2019). Validation of a spectrophotometric methodology for a rapid iodine analysis in algae and seaweed casts. Algal Research, 42, 101613. https://doi.org/10.1016/j.algal.2019.101613pt_PT
dc.identifier.doi10.1016/j.algal.2019.101613pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3158
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectSpectrophotometric validation for rapid iodine content analysis in seaweedspt_PT
dc.subjectIodine variability in collected seaweeds and in seaweed beach castspt_PT
dc.subjectSeaweed applicability in iodine supplementationpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleValidation of a spectrophotometric methodology for a rapid iodine analysis in algae and seaweed castspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIA%2F00329%2F2013/PT
oaire.citation.startPage101613pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume42pt_PT
oaire.fundingStream5876
person.familyNameNunes
person.familyNameFerraz Alves
person.familyNameRoque Lino Felgueiras Barreto
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameNuno
person.givenNameSónia
person.givenNameMaria do Carmo
person.givenNameMiguel Angelo
person.identifierN-8704-2015
person.identifier1218558
person.identifier.ciencia-idD412-34DB-BB84
person.identifier.ciencia-id3716-94D4-BF42
person.identifier.ciencia-idC512-A6FD-4A68
person.identifier.ciencia-id4610-6741-6816
person.identifier.orcid0000-0001-6166-8045
person.identifier.orcid0000-0001-7939-1371
person.identifier.orcid0000-0001-5797-8913
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridAEE-0913-2022
person.identifier.scopus-author-id7004455600
person.identifier.scopus-author-id8758577600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5ac05503-bbdc-4c1e-8bf3-feaed0358e41
relation.isAuthorOfPublicationbbda7eaf-8fdb-4685-8834-31b546e223b5
relation.isAuthorOfPublicationa4511734-ed13-4088-a137-941c7ac0e4f3
relation.isAuthorOfPublication2e433c48-88f8-40a2-b21f-de78b44aab1c
relation.isAuthorOfPublication.latestForDiscoverya4511734-ed13-4088-a137-941c7ac0e4f3
relation.isProjectOfPublicationa2ee6a05-3503-4dd3-8569-f431cc235d08
relation.isProjectOfPublication.latestForDiscoverya2ee6a05-3503-4dd3-8569-f431cc235d08

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Validation of a spectrophotometric methodology for a rapid iodine analysis in algae and seaweed casts.pdf
Size:
345.8 KB
Format:
Adobe Portable Document Format
Download

Collections

Artigos em revistas internacionais