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Improved analytical approach for determination of tropane alkaloids in leafy vegetables based on µ-QuEChERS combined with HPLC-MS/MS

2022-09-20Journal article Open access
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datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.fosCiências Naturais::Ciências Biológicas
dc.contributor.authorGonzález-Gómez, Lorena
dc.contributor.authorMorante-Zarcero, Sonia
dc.contributor.authorPereira, Jorge A. M.
dc.contributor.authorCâmara, José S.
dc.contributor.authorSierra, Isabel
dc.contributor.authorAugusto Machado Pereira, Jorge
dc.date.accessioned2025-06-12T14:01:14Z
dc.date.available2025-06-12T14:01:14Z
dc.date.issued2022-09-20
dc.description.abstract<jats:p>This work presents an optimized methodology based on the miniaturization of the original QuEChERS (μ-QuEChERS) followed by liquid chromatography coupled to mass spectrometry (HPLC-MS/MS) for the determination of tropane alkaloids (TAs), atropine, and scopolamine in leafy vegetable samples. The analytical methodology was successfully validated, demonstrating quantitation limits (MQL) ≤ 2.3 ng/g, good accuracy, and precision, with recoveries between 90–100% and RSD ≤ 13% for both analytes. The method was applied to the analysis of TA-producing plants (Brugmansia versicolor, Solandra maxima, and Convolvulus arvensis). High concentrations of scopolamine were found in flowers (1771 mg/kg) and leaves (297 mg/kg) of B. versicolor. The highest concentration of atropine was found in flowers of S. maxima (10.4 mg/kg). Commercial mixed leafy vegetables contaminated with B. versicolor and S. maxima were analysed to verify the efficacy of the method, showing recoveries between 82 and 110% for both analytes. Finally, the method was applied to the analysis of eighteen samples of leafy vegetables, finding atropine in three samples of mixed leafy vegetables, with concentrations of 2.7, 3.2, and 3.4 ng/g, and in nine samples with concentrations ≤MQL. In turn, scopolamine was only found in a sample of chopped Swiss chard with a concentration ≤MQL.</jats:p>eng
dc.identifier.citationGonzález-Gómez, L.; Morante-Zarcero, S.; Pereira, J.A.M.; Câmara, J.S.; Sierra, I. Improved Analytical Approach for Determination of Tropane Alkaloids in Leafy Vegetables Based on µ-QuEChERS Combined with HPLC-MS/MS. Toxins 2022, 14, 650. https://doi.org/ 10.3390/toxins14100650
dc.identifier.doi10.3390/toxins14100650
dc.identifier.issn2072-6651
dc.identifier.urihttp://hdl.handle.net/10400.13/7301
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.relation.ispartofToxins
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAtropine
dc.subjectScopolamine
dc.subjectTropane alkaloids
dc.subjectµ-QuEChERS
dc.subjectHPLC-MS/MS
dc.subjectLeafy vegetables
dc.subject.
dc.subjectCentro de Química da Madeira
dc.subjectFaculdade de Ciências Exatas e da Engenharia
dc.titleImproved analytical approach for determination of tropane alkaloids in leafy vegetables based on µ-QuEChERS combined with HPLC-MS/MSeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.citation.issue10
oaire.citation.titleToxins
oaire.citation.volume14
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAugusto Machado Pereira
person.givenNameJorge
person.identifier.ciencia-idEC12-EE8F-50E8
person.identifier.orcid0000-0003-0316-5348
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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relation.isProjectOfPublicatione30e13d9-be9a-4f34-91ea-a1682abce74e
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