Percorrer por autor "Silva, Paula Cruz e"
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- Separate and combined effects of cyclic fasting and l-carnitine supplementation in red porgy (Pagrus pagrus, L. 1758)Publication . Nogueira, Natacha; Cordeiro, Nereida; Canada, Paula; Silva, Paula Cruz e; Ozório, Rodrigo O. A.We examined the e¡ects of cyclic fasting in red porgy (Pagrus pagrus) fed di¡erent dietary carnitine levels. Ju venile ¢sh (23.58 3.49 g) were divided into eight groups ^ four groups were fed every day to apparent sa tiation, while the other four were fasted for 7 days every 2 weeks. In each feeding regime, two replicates were fed an L-carnitine non-supplemented diet (46 mg kg 1 ) and the other two groups were fed an L-carnitine sup plemented diet (630 mg kg 1 ). Fish fed 630 mg L-carni tine accumulated two times more L-carnitine in muscle than ¢sh fed 46 mg L-carnitine. Cyclic fasting reduced the growth performance and lipid content in the liver. Carnitine supplementation did not a¡ect performance and body composition, but decreased the n-6 PUFA content. Moreover, the combined e¡ects of fasting and carnitine supplementation were observed on reducing the n-3 fatty acid content. Areas of steatosis were found in the livers of red porgy, but the results revealed that supplementation of L-carnitine in cyclic fasted ¢sh con tributed towards a lower degree of vacuolization than in ¢sh fed to apparent satiation. Regardless of the feed ing regime applied, the spleen of ¢sh fed the L-carni tine-supplemented diet was haemorrhagic and hyper activation of melanomacrophage cells was observed.