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- Characterization of triacylglycerols in Madeira laurel oil by HPLC-atmospheric pressure chemical ionization-MSPublication . Castilho, Paula C.; Costa, Maria do Céu; Rodrigues, Ana; Branco, Pedro Costa; Costa, MafalaaMadeira laurel oil was fractionated by liquid ex traction combined with TLC, and TAGs were analyzed by HPLC coupled with atmospheric pressure chemical ionization-MS (APCI-MS). Eluted molecular species compositions of the eluted TAG in the complex natural mixture were determined by GC identification of FAME and by LC-atmospheric pressure chemi cal ionization (APCI)-MS analysis of the lipid. The APCI-MS spectra of most TAG exhibited [M + H]+ and [M − RCOO]+ ions, which defined the M.W. and the molecular association of fatty acyl residues, respectively. Despite the relatively high de gree of saturation, with a saturated/unsaturated ratio of 0.70, no totally saturated TAG nor mixed asymmetric TAG with two sat urated FA (SSM or SSU, where S is saturated, M is monounsatu rated, and U is unsaturated) were found. This type of molecular structure provides a possible explanation for the relatively low m.p. (12–15°C) and also the high oxidative resistance observed.