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Browsing by Author "Rodrigues, Ana Isabel"

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  • Composition and antimicrobial activity of the essential oil of Clinopodium ascendens (Jordan) Sampaio from Madeira
    Publication . Castilho, Paula; Liu, Kai; Rodrigues, Ana Isabel; Feio, Sónia; Tomi, Félix; Casanova, Joseph
    Clinopodium ascendens (Jordan) Sampaio [synonyms = Calamintha ascendens Jordan = C. officinalis Moench ssp. ascendens (Jord.) Mateo = C. sylvatica Bromf. ssp. ascendens (Jord.) P.W. Ball] is a small herbaceous plant with a very strong and distinctive mint aroma. The plant is known for its medical uses in folk medicine and as a spice in Italian kitchens. In Madeira Island, Clinopodium ascendens, known locally as ‘neveda’, grows wild along the shady paths of the Laurissilva forest. The local population uses the leaves of calamint as a mouth freshener and to alleviate headache and toothache. The essential oil obtained by hydrodistillation of the aerial parts of C. ascendens growing wild in Madeira was analysed by a combination of CC, GC, GC–MS, 1 H- and 13C-NMR. The oil was dominated by C3 oxy genated p-menthane derivatives: cis-isopulegone (75.2%), pulegone (6.9%), neoiso-isopulegol (6.0%) and trans-isopulegone (4.5%). The whole essential oil was tested against a variety of bacteria, both Gram-positive and Gram-negative, and two fungi; it exhibited remarkable activity against Escherichia coli and was active against Agrobacterium tumefasciens and Staphylococcus aureus and the phytopathogenic fungus Botrytis cinerea. It was ineffective against Streptococcus faecium, Streptococcus mutans and Candida albicans.
    2007Journal article Open access Show more
  • Direct identification and quantitative determination of costunolide and dehydrocostuslactone in the fixed oil of Laurus novocanariensis by13C-NMR spectroscopy
    Publication . Ferrari, Bernard; Castilho, Paula; Tomi, Félix; Rodrigues, Ana Isabel; Costa, Maria do Céu; Casanova, Joseph
    The fixed oil of Laurus novocanariensis (previously L. azorica) contains mostly glycerides together with minor non-saponifiable compounds. The direct identification and quantitative determination of costunolide and dehydrocostuslactone, two sesquiterpene lactones components of the oil that exhibit biological activities, is described. The analysis was carried out using 13C-NMR spectroscopy (signal acquisition with inverse gated decoupling of protons; diglyme as internal standard) without separation, derivatisation or any sample preparation.
    2005Journal article Open access Show more