Browsing by Author "Taylor, Gregory J."
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- Evaluating the Madeiran wheat germplasm for aluminum resistance using aluminium-induced callose formation in root apices as a markerPublication . Santos, Teresa M. M. dos; laski, Jan J.; Carvalho, Miguel Â. A. Pinheiro de; Taylor, Gregory J.; Vieira, Maria R. ClementeAluminum (Al) resistance of 57 Madeiran wheat cultivars was evaluated using callose content in root tips and root elongation as markers. Al induced callose formation was a very sensitive indicator of Al damage detecting wide range of genotypic differences existing in the Madeiran wheat germplasm. A weak, yet pos i tive cor re la tion (R2=0.285, P<0.05) between callose content and root elongation was found.
- Factors contributing to the development of aluminum resistance in the Madeiran maize germplasmPublication . Carvalho, Miguel Â. A. Pinheiro de; Slaski, Jan J.; Abreu, Ivo; Ganança, Filipe T.; Santos, Teresa M. M. dos; Freitas, Lurdes; Vieira, Maria R. Clemente; Nunes, Antonieta; Domingues, Ana; Taylor, Gregory J.
- Identification of aluminum resistant genotypes among Madeiran regional wheatsPublication . Carvalho, Miguel Â. A. Pinheiro de; Slaski, Jan J.; Santos, Teresa M. M. dos; Ganança, Filipe T.; Abreu, Ivo; Taylor, Gregory J.; Vieira, Maria R. Clemente; Popova, Tatyana N.; Franco, EmanuelForty-eight genotypes representing wheat diversity from the Island of Madeira were screened for resistance to aluminum (Al) in nutrient solution. Seeds of wheat used in the experiments were obtained from local farmers. The soil pH and content of ionic Al of plots cultivated with wheat were analyzed. The pH of topsoils varied between 3.83 and 6.59. The amount of ionic Al in soil samples varied between 0.38 and 1.36 cmol Al3 + per kg of soil and was positively correlated with the altitude of a plot. Eriochrome cyanine staining was used to evaluate the effect of Al ions on the root elongation. Seventy-two hour exposure of 3-day-old seedlings to 100 and 200 mM Al in nutrient solution revealed a high number of Al resistant genotypes among wheat germplasm. After withdrawal of Al stress, survival and root regrowth was observed in 28 and 23 genotypes screened at 100 and 200 mM Al in nutrient solution, respectively. Enhanced resistance to Al among Madeiran genotypes was associated with the amount of ionic Al in the soils. Complexity and various patterns of responses of tested cultivars to Al stress may suggest that Madeiran germplasm could be a valuable source of genes controlling Al resistance for conventional breeding programs and for studies of molecular bases of mechanisms of Al resistance.