Browsing by Issue Date, starting with "2010-10"
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- Relação entre aptidão física, participação desportiva e comportamentos sedentários e alimentares: um estudo em alunos dos 2º e 3º Ciclos, e Ensino Secundário do Concelho de SantanaPublication . Batista, Juan Gabriel Teixeira; Almeida, Maria JoãoO presente trabalho teve como objectivo caracterizar a população escolar do 5.º ao 12º anos de escolaridade do concelho de Santana, relativamente aos níveis de adiposidade, de aptidão e actividade física, actividades sedentárias e hábitos alimentares. A amostra foi constituída por 505 jovens de ambos os géneros, com idades compreendidas entre os 10 e os 22 anos, distribuídos por quatro grupos etários. A composição corporal foi caracterizada através de: 1) do IMC, com base nos valores de referência de Cole et al. (2000), para determinar excesso de peso e obesidade, e de Cole et al. (2007), para a subnutrição; 2) cálculo da %MG através das pregas subcutâneas segundo as fórmulas de Slaughter et al. (1988), e classificada segundo Lohman (1987). A aptidão física foi avaliada segundo as orientações da bateria de testes Fitnessgram (Cooper Institute for Aerobics Research, 2002). As actividades sedentárias e os hábitos alimentares foram avaliados através de questionários (Sallis et al., 1999 e Wilson et al., 2008 respectivamente), e o grupo de participação desportiva, determinado a partir de questões relativas à prática de actividades desportivas organizadas. Os resultados demonstram uma taxa de prevalência de excesso de peso e obesidade de 26,7%, sendo superior a taxa de prevalência nos participantes do sexo masculino e de idade mais baixa. Ao nível da actividade física organizada extracurricular, verificou-se que 41,5% indicaram praticar outra actividade (DE ou DF), para além das aulas de EF. Relativamente a aptidão física, constatamos que metade da população avaliada, foi classificada abaixo da Zona Saudável de Aptidão Física (ZSAptF), em pelo menos 3, dos 6 testes motores avaliados. A aptidão aeróbia revelou-se como uma das componentes da aptidão com maior taxa de insucesso (54,8%). Ainda na aptidão, verificamos que os participantes classificados abaixo ZSAptF na componente aptidão aeróbia diferiam dos classificados dentro da ZSAptF, nos parâmetros: a) obesidade; b) restantes testes motores da aptidão física avaliados; c) actividades sedentárias (navegar da net; jogo de vídeo portáteis); d) participação desportiva; e) frequência de consumo de bebidas açucaradas, sendo que em média, os alunos classificados abaixo da ZSAptF, apresentam valores superiores de obesidade, piores prestações nos testes motores, menor probabilidade de participarem em actividades organizadas extracurriculares e menor consumo de bebidas açucaradas.
- Encapsulation of single hMSCs in polyelectrolyte shellsPublication . Freitas, Neide Miriam Figueira Correia de; Tomás, Helena; Rodrigues, JoãoThe main objective of this Thesis was to encapsulate single viable cells within polyelectrolyte films using the Layer-by-Layer (LbL) technique. Most of the experiments used human mesenchymal stem cells (MSCs) whose characteristics (capacity of selfrenewal and potential to differentiate into several types of cells) make them particularly interesting to be used in biomedical applications. Also, most of the experiments used alginate (ALG) as the anionic polyelectrolyte and chitosan (CHI) or poly(allylamine hydrochloride) (PAH) as the cationic polyelectrolyte. Hyaluronic acid (HA) was also tested as an anionic polyelectrolyte. At the beginning of the work, the experimental conditions necessary to obtain the encapsulation of individual cells were studied and established. Through fluorescence microscopy visualization by staining the cell nucleus and using polyelectrolytes conjugated to fluorescent dyes, it was possible to prove the obtainment of capsules containing one single cell inside. Capsules aggregation was an observed problem which, despite the efforts to design an experimental process to avoid this situation (namely, by playing with cell concentration and different means of re-suspending and stirring the cells), was not completely overcome. In a second part of the project, single cells were encapsulated within polyelectrolyte layers made of CHI/ALG, PAH/ALG and PAH/HA and their viability was evaluated through the resazurin reduction assay and the Live/Dead assay. In these experiments, during the LbL process, polyelectrolyte solutions were used at a concentration of 1mg/mL based on literature. In general, the viability of the encapsulated cells was shown to be very low/absent. Then, as a consequence of the lack of viability of cells encapsulated within polyelectrolyte layers, the LbL technique was applied in cells growing adherent to the surface of cell culture plates. The cells were cultured like in a sandwich, between the surface of the cell culture dish and the polyelectrolyte layers. Also here, the polyelectrolyte solutions were used at a concentration of 1mg/mL during the LbL process. Surprisingly, cell viability was also absent in these systems. A systematic study (dose-effect study) was performed to evaluate the effect of the concentration of the individual polyelectrolytes (ALG, CHI and PAH were studied) in cell viability. Experiments were performed using cells growing adherent to the surface of cell culture plates. The results pointed out that a very high (cytotoxic) concentration of polyelectrolytes had been in use. Also, in general, PAH was much more cytotoxic than CHI, whereas ALG was the less cytotoxic polyelectrolyte. Finally, using alginate and chitosan solutions with adequate concentrations (low concentrations: 50ng/mL and 1μg/mL), the encapsulation of single viable cells was again attempted. Once again, the encapsulated cells were not shown to be viable. In conclusion, the viability of the encapsulated cells is not only dependent on the cytotoxic characteristics (or combined cytotoxic characteristics) of the polyelectrolytes but it seems that, when detached from the culture plates, the cells become too fragile and lose their viability very easily.