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- Establishment of authenticity and typicality of sugarcane from Madeira Island, and its derivative sugarcane honey, based on molecular, chemical and chemometric analysisPublication . Silva, Pedro Miguel Capêlo da; Câmara, José de Sousa; Nunes, Fernando Hermínio Ferreira MilheiroFood authenticity has become a worldwide concern, foresting the importance of the development of strategies to guarantee the quality and safety of food products. The purpose of this doctoral thesis was the establishment of the typicality and authenticity of sugarcane cultivars from Madeira Island, and its main derivative, sugarcane honey. The followed strategy was based on the identification of typical volatile, sugars and furan derivatives profiles in sugarcane honey processed by the certified producer Fábrica Mel-de-Cana Ribeiro Sêco, and compare it with sugarcane-based syrups from non-certified regional producers and from producers from different geographical regions. Also, the typical volatile profile throughout all stages of sugarcane honey processing were identified during four years, allowing the evaluation of influence of each stage in the formation of its typicality. In addition, the typical volatile profile of all sugarcane cultivars currently cultivated in Madeira Island were identified, namely amarela, canica, roxa, radiada, verde and violeta. Different analytical methodologies based on effective extractive techniques and high-resolution chromatographic methods were successfully developed, optimized and validated, namely solid-phase microextraction combined with gas chromatography-mass spectrometry for the volatile profile, microextraction by packed sorbent combined with ultra-high performance liquid chromatography with diode-array detector for the furan derivatives profile, and ultrasound-assisted liquid-liquid extraction combined with liquid chromatography with a refractive index detector for sugars profile. Furthermore, an innovative and powerful Quality-by-Design approach was selected for development of the methodologies used for quantitation of furan derivatives and SGs. An exhaustive chemometric analysis based on one-way ANOVA, principal component analysis, partial least squares, linear discriminant analysis and hierarchical clustering analysis was successfully applied to recognize the typical profiles of sugarcane honey. This doctoral thesis represents the first attempt to define the typicality, authenticity and traceability of sugarcane honey from Madeira Island, providing a highly valuable information to support its European Union certification.