Artigos em revistas internacionais
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Percorrer Artigos em revistas internacionais por Domínios Científicos e Tecnológicos (FOS) "Ciências Agrárias::Agricultura, Silvicultura e Pescas"
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- Analysis of the environmental impact of botanical pesticides in soilPublication . Pereira, Verónica; Castilho, Paula C.; Pereira, Jorge A. M.; Augusto Machado Pereira, JorgePlant-based pesticides are considered viable complements of conventional synthetic pesticides in agriculture. Their environmentally benign nature and potential to mitigate ecological impacts render them advantageous options for sustainable farming practices. However, the long-term effects of botanical pesticides on soil ecosystems remain unclear. This review aims to examine current evidence concerning the persistence of botanical pesticides in soil environments and their potential effects. Specifically, it addresses their biodegradation pathways in soil as well as their impact on soil enzymes and biology. The methodologies available to perform these studies are also briefly discussed, particularly focusing on how they can be tailored to improve the analysis of the impacts and challenges posed by the use of botanical pesticides in ecosystems.
- Grape pomace as a renewable natural biosource of value-added compounds with potential food industrial applicationsPublication . Abreu, Teresa; Sousa, Patrícia; Gonçalves, Jéssica; Hontman, Nance; Teixeira, Juan; Câmara, José S.; Perestrelo, Rosa; De Abreu Abreu, Maria Teresa; Gonçalves, Jéssica; Câmara, José; Perestrelo, Rosa; Hontman, NanceGrowing consumer demand for environmentally conscious, sustainable, and helpful products has prompted scientists and industry experts worldwide to look for inventive approaches to mitigate the environmental impact, particularly concerning agricultural and industrial waste. Among the by-products of winemaking, grape pomace (skins, seeds, stems) has the potential to be economically valuable as it is rich in value-added compounds (e.g., phenolic compounds, fibers, flavonoids, anthocyanins, terpenoids) related to health (e.g., antioxidant, antimicrobial, anti-inflammatory, cardioprotective effects) and technological issues (e.g., extraction of value-added compounds). These value-added compounds can be extracted using emerging green extraction techniques and then used in the food industry as preservatives, colorants, and for the formulation of functional foods, as well as in the development of smart food packaging. This review provides an overview of the value-added compounds identified in grape pomace, the emerging green extraction, and integrated approaches to extract value-added compounds based on the literature published in the last five years. The potential applications of these value-added compounds have been extensively researched for the food industry.
- Impact of Japanese beetles (Popillia japonica Newman) on the chemical composition of two grape varieties (Nebbiolo and Erbaluce) grown in ItalyPublication . Selli, Serkan; Perestrelo, Rosa; Kelebek, Hasim; Sevindik, Onur; Travaglia, Fabiano; Coïsson, Jean Daniel; Câmara, José S.; Bordiga, Matteo; Perestrelo, Rosa; Câmara, JoséThe effect of Japanese beetles (P. japonica) on critical quality indicators of Nebbiolo and Erbaluce grapes, spe cifically on their phenolic and volatile composition, was assessed. Adult beetle symptoms include extended skeletonization of leaves. Leaves are frequently left with their mid-vein intact but, when severely damaged, quickly turn brown. However, the plant tends to recover by generating a new leaf apparatus and the grapes reach ripeness. It emerged that the phenolic content of grapes produced by plants attacked by P. japonica (396 and 550 mg/kg, Nebbiolo and Erbaluce respectively) was generally higher when compared to healthy plants (266 and 188 mg/kg, Nebbiolo and Erbaluce respectively). Similarly, in the (red) Nebbiolo cultivar, the anthocyanin content was significantly lower in grapes produced with healthy plants. The influence of P. japonica on the volatile composition of Nebbiolo and Erbaluce grapes showed a total volatile fraction of affected grapes (433 and 439 µg/kg, respectively) significantly higher than the one related to healthy grapes (391 and 386 µg/kg, respectively). In response to the attack by P. japonica the plant significantly increases the content of some volatile compounds such as hexanal, (E)-2-hexenal, 1-hexanol, (E)-2-hexen-1-ol and phenyl ethyl alcohol.
- Inhibitory activity of the peel and seed of Annona cherimola Mill. against Fusarium and Aspergillus pathogensPublication . Trindade, Clarissa; Gouveia, Carla S .S.; Oliveira, Maria Cristina O.; Nóbrega, Humberto; Freitas, José G. R. de; Carvalho, Miguel A. A. Pinheiro de; Gouveia, Carla; Oliveira, Cristina; Nóbrega, Humberto; Almeida Pinheiro de Carvalho, Miguel AngeloThe food industry plays a critical role in waste production, and new studies that propose innovative uses to bio-residue are of high importance. Annona cherimola Mill., also known as custard apple and cherimoya, has been described as a powerful source of bioactive compounds with various applications, such as antifungal applications. Fungal infection can lead to crop productivity limitation, product contamination, and food loss. This work evaluates the antifungal action of Annona cherimola Mill. oils against Fusarium oxysporum and Aspergillus niger. The oils were extracted from peel and seeds of 8 different A. cherimola varieties exclusive to Madeira Island (Portugal) and tested for their antifungal potential. This fruit waste was tested, using two different growth measurement methods. The extracted oils restricted the fungi growth, providing the first insights on antifungal properties of the varieties waste. Between the tested tissues, peel oils exhibited a slight better action over F. oxysporum than seed oils. Overall colony expansion of F. oxysporum was better controlled by custard apple oils than A. niger. Anis exhibited distinctive action against A. niger and Funchal, Perry Vidal, Matteus, and Dona Mécia controlled better F. oxysporum growth, suggesting phytochemical differences between them. There is no previous research on the biowaste oil extracts from Annona varieties found in Madeira Island and their properties against pathogens. This study successfully opened new insights on these varieties and the results exposed interesting properties that deserve further research.
- Machine learning system for commercial banana harvestingPublication . Hayat, Ahatsham; Baglat, Preety; Mendonça, Fábio; Mostafa, Sheikh Shanawaz; Morgado-Dias, Fernando; Baglat, Preety; Silva Mendonça, Fábio Rúben; Morgado-Dias, FernandoAbstract The conventional process of visual detection and manual harvesting of the banana bunch has been a known problem faced by the agricultural industry. It is a laborious activity associated with inconsistency in the inspection and grading process, leading to post-harvest losses. Automated fruit harvesting using computer vision empowered by deep learning could significantly impact the visual inspection process domains, allowing consistent harvesting and grading. To achieve the goal of the industry-level harvesting process, this work collects data from professional harvesters from the industry. It investigates six state-of-the-art architectures to find the best solution. 2,685 samples were collected from four different sites with expert opinions from industry harvesters to cut (or harvest) and keep (or not harvest) the banana brunch. Comparative results showed that the DenseNet121 architecture outperformed the other examined architectures, reaching a precision, recall, F1 score, accuracy, and specificity of 85%, 82%, 82%, 83%, and 83%, respectively. In addition, an understanding of the underlying black box nature of the solution was visualized and found adequate. This visual interpretation of the model supports human expert’s criteria for harvesting. This system can assist or replace human experts in the field.
- Microorganism community structure: a characterisation of agrosystems from Madeira ArchipelagoPublication . Oliveira, Maria Cristina O.; Ragonezi, Carla; Valente, Sofia; Freitas, José G. R. de; Carvalho, Miguel A. A. Pinheiro de; Oliveira, Cristina; Ragonezi, Carla; Valente, Sofia; Almeida Pinheiro de Carvalho, Miguel AngeloAbstract Microbial diversity profoundly influences soil ecosystem functions, making it vital to monitor community dynamics to comprehend its structure. Our study focused on six agrosystems in Madeira Archipelago, analysing bacteria, archaea, fungi and AMF through classical microbiology and molecular techniques. Despite distinct edaphoclimatic conditions and management practices, bacterial structures exhibited similarities, with Alphaproteobacteria at 18%–20%, Bacilli at 11%–18% and Clostridia at 9%–14%. The predominance of copiothrophic groups suggested that soil nutrient content was the driver of these communities. Regarding archaea, the communities changed among sites, and it was evident that agrosystems provided niches for methanogens. The Crenarchaeota varied between 15% and 29%, followed by two classes of Euryarchaeota, Methanomicrobia (17%–25%) and Methanococci (4%–32%). Fungal communities showed consistent composition at the class level but had differing diversity indices due to management practices and soil texture. Sordaryomycetes (21%–28%) and Agaricomycetes (15%–23%) were predominant. Conversely, AMF communities appeared to be also influenced by the agrosystem, with Glomus representing over 50% of the community in all agrosystems. These insights into microbial groups' susceptibilities to environmental conditions are crucial for maintaining healthy soil and predicting climate change effects on agrosystems' productivity, resilience and sustainability. Additionally, our findings enable the development of more robust prediction models for agricultural practices.
- Organic farming enhances diversity and recruits beneficial soil fungal groups in traditional banana plantationsPublication . Oliveira, Maria Cristina O.; Alves, Artur; Ragonezi, Carla; Freitas, José G. R. de; Carvalho, Miguel A. A. Pinheiro de; Oliveira, Cristina; Ragonezi, Carla; Almeida Pinheiro de Carvalho, Miguel AngeloThis study investigates the impact of organic (OF) and conventional farming (CF) on soil fungal communities in banana monoculture plantations on Madeira Island. We hypothesized that OF promotes beneficial fungal groups over harmful ones, sustaining soil health. Soil samples were collected from six plantations (three OF and three CF) for ITS amplicon sequencing to assess fungal diversity. Results showed that OF significantly enhanced fungal alpha-diversity (Shannon–Wiener index) and Evenness. The phylum Ascomycota dominated OF systems, while Basidiomycota prevailed in CF. Mortierella, a beneficial genus, was abundant in OF and is observed in CF but was less evident, being the genus Trechispora the most well represented in CF agrosystems. Additionally, OF was associated with higher soil pH and Mg levels, which correlated positively with beneficial fungal groups. Functional analysis revealed that OF promoted saprotrophic fungi, crucial for the decomposition of organic matter and nutrient cycling. However, both systems exhibited low levels of arbuscular mycorrhizal fungi, likely due to high phosphorus levels. These findings suggest that organic practices can enhance soil fungal diversity and health, although attention to nutrient management is critical to further improving soil–plant–fungi interactions.
- O projecto Interfruta II e os problemas fitossanitários que afectam as fruteiras na ilha Terceira, AçoresPublication . Lopes , D. J. H.; Cabrera, R.; Aguin-Pombo, D.; Aguin-Pombo, Dora; Borges, P.; Pimentel, R.; Zorman, M.; Macedo, N.; Carvalho., M. C. F.; Ornelas, L.; Martins, J. T.; Mumford, J. D.; Mexia, A. M. M.O Projecto Interfruta II é um projecto apoiado pelo programa Interreg III-B, desenvolvido nas Ilhas da Madeira, Tenerife (Canárias) e Terceira (Açores) destinado a contribuir para a promoção da fruticultura e viticultura nestas três regiões insulares, aplicando técnicas que contribuam decisivamente para o conhecimento e procura de soluções, numa vertente de prospecção das pragas-chave, fauna auxiliar, doenças e vírus que afectam essas culturas. Dentro dos problemas fitossanitários que afectam as culturas estudadas, através da identificação das pragas-chave de cada cultura foi utilizada a observação visual e a monitorização através de armadilhas com feromona sexual e placas cromotrópicas. Na prospecção da fauna auxiliar foi utilizada a técnica das pancadas e a armadilha Malaise. Após a identificação das pragas-chave, centrou-se a investigação sobre as mais importantes. Na mosca-do-Mediterrâneo (C. capitata Wied.), usando os SIG, foi montada uma rede de armadilhas em toda a ilha. Na bananeira, centrou-se a investigação no gorgulho-da-bananeira (Cosmopolitus sordidus Germar) e nas tripes. No castanheiro, o bichado-da-castanha (Cydia splendana Hubner) procedeu-se à sua monitorização utilizando armadilhas com feromona sexual e a quantificação dos prejuízos que causa nos frutos. Nas macieiras, os principais problemas decorrem da presença de aranhiço vermelho (Panonychus ulmi Koch), traça-oriental (Cydia molesta Busck), bichado (Cydia pomonella L.), afídeos e tripes. Os resultados obtidos permitiram construir uma base de dados fitossanitários de diagnóstico da Macaronésia (PROFITOMAC) que se encontra disponível on-line em www.interfruta.net Neste trabalho apenas se apresentam os resultados obtidos nos estudos realizados sobre as pragas e fauna auxiliar.
- Tracing the volatilomic fingerprint of the most popular italian fortified winesPublication . Jasmins, Gonçalo; Perestrelo, Rosa; Coïsson, Jean Daniel; Sousa, Patrícia; Teixeira, José A.; Bordiga, Matteo; Câmara, José S.; Jasmins, Gonçalo; Perestrelo, Rosa; Câmara, JoséThe aim of the current study was to provide a useful platform to identify characteristic molecular markers related to the authenticity of Italian fortified wines. For this purpose, the volatilomic fingerprint of the most popular Italian fortified wines was established using headspace solid-phase microextraction combined with gas chromatography–mass spectrometry (HS-SPME/GC-MS). Several volatile organic compounds (VOCs), belonging with distinct chemical groups, were identified, ten of which are common to all the analyzed fortified Italian wines. Terpenoids were the most abundant chemical group in Campari bitter wines due to limonene’s high contribution to the total volatilomic fingerprint, whereas for Marsala wines, alcohols and esters were the most predominant chemical groups. The fortified Italian wines VOCs network demonstrated that the furanic compounds 2-furfural, ethyl furoate, and 5-methyl-2-furfural, constitute potential molecular markers of Marsala wines, while the terpenoids nerol, α-terpeniol, limonene, and menthone isomers, are characteristic of Vermouth wines. In addition, butanediol was detected only in Barolo wines, and β-phellandrene and β-myrcene only in Campari wines. The obtained data reveal an adequate tool to establish the authenticity and genuineness of Italian fortified wines, and at the same time constitute a valuable contribution to identify potential cases of fraud or adulteration to which they are subject, due to the high commercial value associated with these wines. In addition, they contribute to the deepening of scientific knowledge that supports its valorization and guarantee of quality and safety for consumers.
- Variations in the structure and function of the soil fungal communities in the traditional cropping systems from Madeira IslandPublication . Oliveira, Maria Cristina O.; Alves, Artur; Fidalgo, Cátia; Freitas, José G. R. de; Carvalho, Miguel A. A. Pinheiro de; Oliveira, Cristina; Almeida Pinheiro de Carvalho, Miguel AngeloAgricultural soils are responsible for ecological functions and services that include primary production of food, fiber and fuel, nutrient cycling, carbon cycling and storage, water infiltration and purification, among others. Fungi are important drivers of most of those ecosystem services. Given the importance of fungi in agricultural soils, in this study, we aimed to characterize and analyse the changes of the soil fungal communities of three cropping systems from Madeira Island, where family farming is predominant, and investigate the response of fungi and its functional groups to soil physicochemical properties. To achieve that, we sequenced amplicons targeting the internal transcribed spacer 1 (ITS1) of the rRNA region, to analyse soil samples from 18 agrosystems: 6 vineyards (V), 6 banana plantations (B) and 6 vegetable plantations (H). Our results showed that alpha diversity indices of fungal communities are similar in the three cropping systems, but fungal composition and functional aspects varied among them, with more pronounced differences in B. Ascomycota, Basidiomycota, and Mortierellomycota were the main phyla found in the three cropping systems. Agaricomycetes and Sordariomycetes are the predominant classes in B, representing 23.8 and 22.4%, respectively, while Sordariomycetes (27.9%) followed by Eurotiomycetes (12.3%) were the predominant classes in V and Sordariomycetes (39.2%) followed by Tremellomycetes (8.9%) in the H. Saprotrophs are the fungal group showing higher relative abundance in the three cropping systems, followed by plant pathogens. Regarding symbionts, endophytes were highly observed in B, while mycorrhizal fungi was predominant in V and H. The structure of fungal communities was mainly correlated with soil content of P, K, N, Fe, and Cu. In addition, we identified bioindicators for each cropping system, which means that cultivated crops are also drivers of functional groups and the composition of communities. Overall, the three cropping systems favored diversity and growth of taxa that play important roles in soil, which highlights the importance of conservative management practices to maintain a healthy and resilient agrosystem.