Universidade da Madeira
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Browsing Universidade da Madeira by Field of Science and Technology (FOS) "Ciências Agrárias::Ciência Animal e dos Laticínios"
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- Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatographyPublication . Antos, Joanna; García-Cansino, Laura; Ángeles García, María; Ginter-Kramarczyk, Dobrochna; Marina, María Luisa; Zembrzuska, Joanna; Câmara, José S.; Pereira, Jorge A. M.; Câmara, José; Augusto Machado Pereira, JorgeA μSPEed microextraction combined with ultra-performance liquid chromatography (UHPLC) with UV detection was developed for analysing six veterinary antibiotics (tetracycline, chlortetracycline, oxytetracycline, doxycycline, sulfamethoxazole, and trimethoprim) in environmental samples. To optimise extraction, 12 sorbent cartridges, sample loading cycles, volumes, and pH were assayed. The PS/DVB-RP cartridge, three 250 μL sample loading cycles, and two 50-µL elutions with acidified methanol yielded maximum efficiency. The method was validated with optimised fast chromatographic separation, showing good linearity (R2 > 0.99), precision (RSD < 20%), and recoveries between 46-86%. Detection and quantification limits ranged from 0.30-1.23 μg L−1 and 0.92-3.73 μg L−1 , respectively. The optimised μSPEed/UPLC-PDA efficiently analysed environmental water samples, requiring only 6 min extraction, 6 min analysis, and 500 μL sample, surpassing alternative methods in speed, workloads and reproducibility. The cost-effective, commercially available equipment facilitates accessibility for laboratories and adaptability for analysing selected antibiotics in diverse matrices, including food and environmental samples.
- Unravelling the potential of insects for medicinal purposes: a comprehensive reviewPublication . Siddiqui, Shahida Anusha; Li, Chujun; Aidoo, Owusu Fordjour; Fernando, Ito; Haddad, Moawiya A.; Pereira, Jorge A.M.; Blinov, Andrey; Golik, Andrey; Câmara, José S.; Augusto Machado Pereira, Jorge; Câmara, JoséEntomotherapy, the use of insects for medicinal purposes, has been practised for centuries in many countries around the world. More than 2100 edible insect species are eaten by humans, but little is known about the possibility of using these insects as a promising alternative to traditional pharmaceuticals for treating diseases. This review offers a fundamental understanding of the therapeutic applications of insects and how they might be used in medicine. In this review, 235 insect species from 15 orders are reported to be used as medicine. Hymenoptera contains the largest medicinal insect species, followed by Coleoptera, Orthoptera, Lepidoptera, and Blattodea. Scientists have examined and validated the potential uses of insects along with their products and by-products in treating various diseases, and records show that they are primarily used to treat digestive and skin disorders. Insects are known to be rich sources of bioactive compounds, explaining their therapeutic features such as anti-inflammatory, antimicrobial, antiviral, and so on. Challenges associated with the consumption of insects (entomophagy) and their therapeutic uses include regulation barriers and consumer acceptance. Moreover, the overexploitation of medicinal insects in their natural habitat has led to a population crisis, thus necessitating the investigation and development of their mass-rearing procedure. Lastly, this review suggests po tential directions for developing insects used in medicine and offers advice for scientists inter ested in entomotherapy. In future, entomotherapy may become a sustainable and cost-effective solution for treating various ailments and has the potential to revolutionize modern medicine.
- Valorization of agri-food waste and by-products in cheese and other dairy foods: an updated reviewPublication . Tarchi, Ines; Boudalia, Sofiane; Ozogul, Fatih; Câmara, José S.; Bhat, Zuhaib F.; Hassoun, Abdo; Perestrelo, Rosa; Bouaziz, Mohamed; Nurmilah, Siti; Cahyana, Yana; Aït-Kaddour, Abderrahmane; Perestrelo, RosaFood waste and by-products have attracted attention in recent years due to the reduction of food resources and the negative impact on the environment of current production and consumption systems. However, food waste and by-products can be transformed into new products that can be useful for human consumption because they represent a source of nutritional and technological (e.g., preservative, colorant, fibres) added-value compounds. Those compounds of interest can be extracted using several advanced and/or emerging extraction techniques, which follow the green chemistry principles, and incorporated into food products. This is true for many types of foods; even dairy products of which cheese is one of the most appreciated and consumed worldwide. Therefore, cheese and dairy products can be effective vehicles for food waste and food by-products valorization. This review focuses on the opportunities for valuing various food wastes in the dairy sector, especially in cheese. It also highlights the contributions of recovered bioactive added-value compounds in enhancing the nutritional, technological, and sensory properties of dairy products. It is expected that food wastes could be used to improve functionalities of dairy products in the future, leading to innovative functional dairy food products to decrease the environmental footprint and simultaneously contribute to improve sustainability in the food sector.