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- Identification of endangered petrel species from poor quality feather debris using cytochrome b sequencesPublication . Gonçalves, Rita; Parik, Jüri; Freitas, Ana Isabel; Jesus, José; Villems, Richard; Brehm, António
- Microsatellite variability in natural populations of the blackspot seabream Pagellus bogaraveo (Brunnick, 1768): a database to access parentage assignment in aquaculturePublication . Lemos, Andreia; Freitas, Ana Isabel; Fernandes, Ana Teresa; Gonçalves, Rita; Jesus, José; Andrade, Carlos; Brehm, AntónioEight microsatellite loci previously reported were as sessed for their utility in parentage assignment in 96 individuals belonging to natural populations of the blackspot seabream Pagellus bogaraveo (Brˇnnick, 1768) from the Mediterranean and Northeast Atlantic regions. At the mtDNA level, no di¡erentiation was found between these two regions but based on micro satellite data an overall discrete genetic di¡erentiation is perceivable between the two regions separated by the Strait of Gibraltar. The number of alleles per locus ranged from 8 to 30. A database constructed with al lele frequency data from six populations was used in a simulation parentage assignment test using the software CERVUS. The test showed that the number of markers used is enough to perform parentage assign ments with real data. The polymorphic information content for each locus was very high (mean value of 0.849), with a total exclusionary power of 0.9995. In summary, seven of the eight microsatellites analysed proved to be su⁄cient and powerful tools for parent age assignment in hatcheries and the allele frequency data given here can be used to perform pedigree ana lysis against which real data may be tested.
- Structure and genetic variation of the mitochondrial control region in the honey bee Apis melliferaPublication . Gonçalves, Rita; Freitas, Ana Isabel; Jesus, José; De la Rúa, Pilar; Brehm, AntonioAlthough the mitochondrial molecule of the honey bee is completely sequenced, the control region is rarely used for genetic inferences as in other invertebrates, due to several constraints mainly a biased A plus T content and extensive variable length repeats. Herein, we have analyzed the control region of honey bees from the Iberian Peninsula and North Atlantic islands. The information retrieved when comparing individuals from different populations was crucial to understand and characterize how the control region is organized in this species. As expected, this region in Apis mellifera appears to contain valuable although limited genetic information at the population level. Furthermore, the comparisons of the A. mellifera control region with other species of the same genus highlight the structural role of particular sequences within the A+T rich control region as proposed here.