Microsatellite variability in natural populations of the blackspot seabream Pagellus bogaraveo (Brunnick, 1768): a database to access parentage assignment in aquaculture

Lemos, Andreia; Freitas, Ana Isabel; Fernandes, Ana Teresa; Gonçalves, Rita; Jesus, José; Andrade, Carlos; Brehm, António

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Authors

Lemos, Andreia

Freitas, Ana Isabel

Fernandes, Ana Teresa

Abstract(s)

Eight microsatellite loci previously reported were as sessed for their utility in parentage assignment in 96 individuals belonging to natural populations of the blackspot seabream Pagellus bogaraveo (Brˇnnick, 1768) from the Mediterranean and Northeast Atlantic regions. At the mtDNA level, no di¡erentiation was found between these two regions but based on micro satellite data an overall discrete genetic di¡erentiation is perceivable between the two regions separated by the Strait of Gibraltar. The number of alleles per locus ranged from 8 to 30. A database constructed with al lele frequency data from six populations was used in a simulation parentage assignment test using the software CERVUS. The test showed that the number of markers used is enough to perform parentage assign ments with real data. The polymorphic information content for each locus was very high (mean value of 0.849), with a total exclusionary power of 0.9995. In summary, seven of the eight microsatellites analysed proved to be su⁄cient and powerful tools for parent age assignment in hatcheries and the allele frequency data given here can be used to perform pedigree ana lysis against which real data may be tested.

Keywords

Pagellus bogaraveo Microsatellites Genetic structure Parentage Database . Faculdade de Ciências da Vida

URI

http://hdl.handle.net/10400.13/3678

Citation

Lemos, A., Freitas, A. I., Fernandes, A. T., Gonçalves, R., Jesus, J., Andrade, C., & Brehm, A. (2006). Microsatellite variability in natural populations of the blackspot seabream Pagellus bogaraveo (Brünnick, 1768): a database to access parentage assignment in aquaculture. Aquaculture Research, 37(10), 1028-1033.