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Application of quality-by-design approach in the analytical method development for quantification of sugars in sugarcane honey by reversed-phase liquid chromatography

2020Journal article Open access
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dc.contributor.authorSilva, Pedro
dc.contributor.authorSilva, Catarina L.
dc.contributor.authorPerestrelo, Rosa
dc.contributor.authorNunes, Fernando M.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2022-01-04T15:30:35Z
dc.date.available2022-01-04T15:30:35Z
dc.date.issued2020
dc.description.abstractProduced in Madeira Island from regional sugarcane cultivars through a traditional manufacturing and storage process, sugarcane honey (SCH) is a black syrup recognized by its excellent quality. Its economic value has led to the emergence of adulterated SCH, whereby the identification of molecular markers became an essential task in order to overcome the fraudulent activities, protect its authenticity, and guarantee the consumer safety. In the present study, an analytical strategy based on ultrasound-assisted liquid liquid extraction (USA-LLE) followed by reversed-phase liquid chromatography with a refractive index detector (LC-RI) was developed for the determination of sugars (glucose, fructose, sucrose, xylose, and mannose) in SCH samples from certified producers, supported on analytical quality-by-design (AQbD) approach, as a useful tool to establish its typicality. The application of AQbD was based on analytical risk assessment, multivariate statistics and quality control procedures for definition of the Method Operable Design Region (MODR). The optimal conditions into MODR were accomplished using BEH Amide column operating at a temperature of 80 °C and a flow rate of 300 μL min−1 , with a mobile phase composed by acetone and water (85:15, v v−1 ) at a flow rate of 1.0 mL min−1 . The robustness was determined by Monte Carlo simulation and capability analysis. The concentration-response function for all sugars was described by polynomial models. Accuracy was presented by recovery values between 98.2 and 119.5%. The analytical figures of merit validated the utility of AQbD in the systematic design of a LC-RI method with fine sensitivity for sugar analysis in SCH.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, P., Silva, C. L., Perestrelo, R., Nunes, F. M., & Câmara, J. S. (2020). Application of quality-by-design approach in the analytical method development for quantification of sugars in sugarcane honey by reversed-phase liquid chromatography. Food Analytical Methods, 13, 1634-1649. https://doi.org/10.1007/s12161-020-01767-7pt_PT
dc.identifier.doi10.1007/s12161-020-01767-7pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3945
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSugarcane honeypt_PT
dc.subjectSugarpt_PT
dc.subjectTypicalitypt_PT
dc.subjectQuality-by-designpt_PT
dc.subjectUSA-LLEpt_PT
dc.subjectLC-RIpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleApplication of quality-by-design approach in the analytical method development for quantification of sugars in sugarcane honey by reversed-phase liquid chromatographypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.citation.endPage1649pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1634pt_PT
oaire.citation.titleFood Analytical Methodspt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCapêlo da Silva
person.familyNameSousa Luís
person.familyNamePerestrelo
person.familyNameNunes
person.familyNameCâmara
person.givenNamePedro Miguel
person.givenNameCatarina Grace
person.givenNameRosa
person.givenNameFernando
person.givenNameJosé
person.identifierC-1300-2019
person.identifier1441319
person.identifier60597
person.identifierG-3003-2013
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person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0003-1487-9346
person.identifier.orcid0000-0002-3018-3165
person.identifier.orcid0000-0002-7223-1022
person.identifier.orcid0000-0001-5540-318X
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridI-2307-2014
person.identifier.ridB-3003-2012
person.identifier.scopus-author-id57194492726
person.identifier.scopus-author-id16686828800
person.identifier.scopus-author-id7102392847
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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