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Zwitterion-functionalized dendrimer-entrapped gold nanoparticles for serum-enhanced gene delivery to inhibit cancer cell metastasis

2019Journal article Open access
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dc.contributor.authorXiong, Zhijuan
dc.contributor.authorAlves, Carla S.
dc.contributor.authorWang, Jianhua
dc.contributor.authorLi, Aijun
dc.contributor.authorLiu, Jinyuan
dc.contributor.authorShen, Mingwu
dc.contributor.authorRodrigues, João
dc.contributor.authorTomás, Helena
dc.contributor.authorShi, Xiangyang
dc.date.accessioned2022-03-08T11:47:29Z
dc.date.available2022-03-08T11:47:29Z
dc.date.issued2019
dc.description.abstractWe demonstrate a novel serum-enhanced gene delivery approach using zwitterion-functionalized dendrimer-entrapped gold nanoparticles (Au DENPs) as a non-viral vector for inhibition of cancer cell metastasis in vitro. Poly(amidoamine) dendrimers of generation 5 decorated with zwitterion carboxybe taine acrylamide (CBAA) and lysosome-targeting agent morpholine (Mor) were utilized to entrap gold NPs. We show that both Mor-modified and Mor-free Au DENPs are cytocompatible and can effectively deliver plasmid DNA encoding different reporter genes to cancer cells in medium with or without serum. Strikingly, due to the antifouling property exerted by the attached zwitterion CBAA, the gene delivery efficiency of Mor-modified Au DENPs and the Mor-free Au DENPs in the serum-containing medium are 1.4 and 1.7 times higher than the corresponding vector in serum-free medium, respectively. In addition, the Mor-free vector has a better gene expression efficiency than the Mor-modified one although the Mor modification enables the polyplexes to have enhanced cancer cell uptake. Wound healing and hyperme thylated in cancer 1 (HIC1) protein expression assay data reveal that the expression of HIC1 gene in cancer cells enables effective inhibition of cell migration. Our findings suggest that the created zwitterion-functionalized Au DENPs may be employed as a powerful vector for serum-enhanced gene therapy of different diseases.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationXiong, Z., Alves, C. S., Wang, J., Li, A., Liu, J., Shen, M., ... & Shi, X. (2019). Zwitterion-functionalized dendrimer-entrapped gold nanoparticles for serum-enhanced gene delivery to inhibit cancer cell metastasis. Acta Biomaterialia, 99, 320-329. https://doi.org/10.1016/j.actbio.2019.09.005pt_PT
dc.identifier.doi10.1016/j.actbio.2019.09.005pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4124
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectHIC1 genept_PT
dc.subjectDendrimerspt_PT
dc.subjectZwitterionspt_PT
dc.subjectGold nanoparticlespt_PT
dc.subjectGene deliverypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleZwitterion-functionalized dendrimer-entrapped gold nanoparticles for serum-enhanced gene delivery to inhibit cancer cell metastasispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.citation.endPage329pt_PT
oaire.citation.startPage320pt_PT
oaire.citation.titleActa Biomaterialiapt_PT
oaire.citation.volume99pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAlves
person.familyNameRodrigues
person.familyNameTomás
person.familyNameShi
person.givenNameCarla Sophia
person.givenNameJoão
person.givenNameHelena
person.givenNameXiangyang
person.identifier556975
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.ciencia-idBA11-1437-B948
person.identifier.orcid0000-0002-8891-5234
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-6785-6645
person.identifier.ridB-6816-2008
person.identifier.ridE-5991-2010
person.identifier.ridA-1289-2007
person.identifier.scopus-author-id11438813200
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id6508104177
person.identifier.scopus-author-id7402953116
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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