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PAMAM dendrimer/pDNA functionalized-magnetic iron oxide nanoparticles for gene delivery

2015Journal article Restricted access
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dc.contributor.authorXiao, Shili
dc.contributor.authorCastro, Rita
dc.contributor.authorRodrigues, João
dc.contributor.authorShi, Xiangyang
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-06-24T11:03:20Z
dc.date.available2019-06-24T11:03:20Z
dc.date.issued2015
dc.description.abstractHerein, we report an easy and ingenious method to functionalize magnetic iron oxide nanoparticles (MNPs) with plasmid DNA (pDNA) to obtain nanohybrid systems suitable for nucleic acid therapy. The nanohybrids were prepared by combining complexes of dendrimers and pDNA (dendriplexes) and poly(styrene) sulfonate-coated MNPs through electrostatic interactions. The effects of the dendrimer generation (generations 2, 4 and 6) and the amine to phosphate group (N/P) ratio on the hydrodynamic diameter, zeta potential, cell viability, cellular internalization and transfection efficiency of the nanohybridswere systematically investigated at different transfection conditions (including incubation time, pDNA concentration, presence or absence of an external magnetic field, and presence or absence of fetal bovine serum). The results confirmed that the nanohybrids were able to transfect NIH 3T3 cells, and that the level of gene expression (the luciferase protein reporter gene was used) was strongly dependent on the dendrimer generation, the N/P ratio, and the pDNA concentration. The best system was based on dendriplex-coated MNPs formed by generation 6 dendrimers at an N/P ratio of 10 that, at optimized conditions, led to a gene expression level which was not significantly different from that obtained only using dendriplexes. In summary, a coherent set of results was reached indicating the potential of the developed nanohybrids as effective gene delivery nanomaterials.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationXiao, S., Castro, R., Rodrigues, J., Shi, X., & Tomás, H. (2015). PAMAM dendrimer/pDNA functionalized-magnetic iron oxide nanoparticles for gene delivery. Journal of biomedical nanotechnology, 11(8), 1370-1384.pt_PT
dc.identifier.doi10.1166/jbn.2015.2101pt_PT
dc.identifier.issn1550-7033
dc.identifier.urihttp://hdl.handle.net/10400.13/2422
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Scientific Publisherspt_PT
dc.relationNEW MATERIALS FOR BIOMEDICAL APPLICATIONS OBTAINED BY THE SELF-ASSEMBLY OF DENDRIMER-SINGLE STRANDED DNA CONJUGATES
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectPoly(amidoamine) dendrimerpt_PT
dc.subjectMagnetic nanoparticlept_PT
dc.subjectIron oxidept_PT
dc.subjectNanohybridpt_PT
dc.subjectGene deliverypt_PT
dc.subjectTransfectionpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titlePAMAM dendrimer/pDNA functionalized-magnetic iron oxide nanoparticles for gene deliverypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNEW MATERIALS FOR BIOMEDICAL APPLICATIONS OBTAINED BY THE SELF-ASSEMBLY OF DENDRIMER-SINGLE STRANDED DNA CONJUGATES
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F75420%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-NAN%2F112428%2F2009/PT
oaire.citation.endPage1384pt_PT
oaire.citation.startPage1370pt_PT
oaire.citation.titleJournal of Biomedical Nanotechnologypt_PT
oaire.citation.volume11(8)pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNameXiao
person.familyNameCastro
person.familyNameRodrigues
person.familyNameShi
person.familyNameTomás
person.givenNameShili
person.givenNameRita
person.givenNameJoão
person.givenNameXiangyang
person.givenNameHelena
person.identifier556975
person.identifier.ciencia-id8314-881E-4986
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-idBA11-1437-B948
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0001-9955-2064
person.identifier.orcid0000-0002-9337-062X
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0001-6785-6645
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridA-3537-2013
person.identifier.ridA-2497-2013
person.identifier.ridB-6816-2008
person.identifier.ridA-1289-2007
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id35192467200
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id7402953116
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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