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Electrospun laponite-doped poly(lactic-co-glycolic acid) nanofibers for osteogenic differentiation of human mesenchymal stem cells

2012Journal article Restricted access
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dc.contributor.authorWang, Shige
dc.contributor.authorCastro, Rita
dc.contributor.authorAn, Xiao
dc.contributor.authorSong, Chenlei
dc.contributor.authorLuo, Yu
dc.contributor.authorShen, Mingwu
dc.contributor.authorTomás, Helena
dc.contributor.authorZhu, Meifang
dc.contributor.authorShi, Xiangyang
dc.date.accessioned2019-07-09T13:23:08Z
dc.date.available2019-07-09T13:23:08Z
dc.date.issued2012
dc.description.abstractWe report the fabrication of uniform electrospun poly(lactic-co-glycolic acid) (PLGA) nanofibers incorporated with laponite (LAP) nanodisks, a synthetic clay material for osteogenic differentiation of human mesenchymal stem cells (hMSCs). In this study, a solution mixture of LAP suspension and PLGA was electrospun to form composite PLGA–LAP nanofibers with different LAP doping levels. The PLGA–LAP composite nanofibers formed were systematically characterized via different techniques. We show that the incorporation of LAP nanodisks does not significantly change the uniform PLGA fiber morphology, instead significantly improves the mechanical durability of the nanofibers. Compared to LAP-free PLGA nanofibers, the surface hydrophilicity and protein adsorption capacity of the composite nanofibers slightly increase after doping with LAP, while the hemocompatibility of the fibers does not appreciably change. The cytocompatibility of the PLGA–LAP composite nanofibers was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of L929 mouse fibroblasts and porcine iliac artery endothelial cells cultured onto the surface of the nanofibers. The results reveal that the incorporated LAP is beneficial to promote the cell adhesion and proliferation to some extent likely due to the improved surface hydrophilicity and protein adsorption capability of the fibers. Finally, the PLGA–LAP composite nanofibers were used as scaffolds for osteogenic differentiation of hMSCs. We show that both PLGA and PLGA–LAP composite nanofibers are able to support the osteoblast differentiation of hMSCs in osteogenic medium. Most strikingly, the doped LAP within the PLGA nanofibers is able to induce the osteoblast differentiation of hMSCs in growth medium without any inducing factors. The fabricated smooth and uniform organic–inorganic hybrid LAP-doped PLGA nanofibers may find many applications in the field of tissue engineering.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationWang, S., Castro, R., An, X., Song, C., Luo, Y., Shen, M., ... & Shi, X. (2012). Electrospun laponite-doped poly(lactic-co-glycolic acid) nanofibers for osteogenic differentiation of human mesenchymal stem cells. Journal of Materials Chemistry, 22(44), 23357-23367.pt_PT
dc.identifier.doi10.1039/c2jm34249apt_PT
dc.identifier.issn0959-9428
dc.identifier.urihttp://hdl.handle.net/10400.13/2467
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectElectrospun laponite-doped poly(lactic-co-glycolic acid) nanofiberspt_PT
dc.subjectOsteogenic differentiationpt_PT
dc.subjectHuman mesenchymal stem cellspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleElectrospun laponite-doped poly(lactic-co-glycolic acid) nanofibers for osteogenic differentiation of human mesenchymal stem cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage23367pt_PT
oaire.citation.startPage23357pt_PT
oaire.citation.titleJournal of Materials Chemistrypt_PT
oaire.citation.volume22(44)pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameWang
person.familyNameCastro
person.familyNameTomás
person.familyNameShi
person.givenNameShige
person.givenNameRita
person.givenNameHelena
person.givenNameXiangyang
person.identifier556975
person.identifier.ciencia-id8314-881E-4986
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.ciencia-idBA11-1437-B948
person.identifier.orcid0000-0002-7639-6035
person.identifier.orcid0000-0002-9337-062X
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-6785-6645
person.identifier.ridA-2497-2013
person.identifier.ridE-5991-2010
person.identifier.ridA-1289-2007
person.identifier.scopus-author-id53882129600
person.identifier.scopus-author-id6508104177
person.identifier.scopus-author-id7402953116
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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