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An improved analytical strategy combining microextraction by packed sorbent combined with ultra high pressure liquid chromatography for the determination of fluoxetine, clomipramine and their active metabolites in human urine

2015Journal article Restricted access
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dc.contributor.authorAlves, Vera
dc.contributor.authorGonçalves, João
dc.contributor.authorConceição, Carlota
dc.contributor.authorTeixeira, Helena M.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-23T10:58:40Z
dc.date.available2019-07-23T10:58:40Z
dc.date.issued2015
dc.description.abstractA powerful and sensitive method, by microextraction packed sorbent (MEPS), and ultra-high performance liquid chromatography (UHPLC) with a photodiode array (PDA) detection, is described for the determination of fluoxetine, clomipramine and their active metabolites in human urine samples. The MEPS variables, such as sample volume, pH, number of extraction cycles (draw-eject), and desorption conditions (solvent and solvent volume of elution) were optimized. The analysis were carried out using small sample volumes (500μL) and in a short time period (5min for the entire sample preparation step). Good linearity was obtained for all antidepressants with the correlation coefficients (R(2)) above 0.9965. The limits of detection (LOD) ranged from 0.068 to 0.087μgmL(-1). The recoveries were from 93% to 98%, with relative standard deviations less than 6%. The inter-day precision, expressed as the relative standard deviation, varied between 3.8% and 8.5% while the intra-day precision between 3.0% and 7.1%. In order to evaluate the proposed method for clinical use, the MEPS/UHPLC-PDA method was applied to analysis of urine samples from depressed patients.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlves, V., Gonçalves, J., Conceição, C., Teixeira, H. M., & Câmara, J. S. (2015). An improved analytical strategy combining microextraction by packed sorbent combined with ultra high pressure liquid chromatography for the determination of fluoxetine, clomipramine and their active metabolites in human urine. Journal of Chromatography A, 1408, 30-40.pt_PT
dc.identifier.doi10.1016/j.chroma.2015.07.021pt_PT
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10400.13/2499
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectAntidepressantspt_PT
dc.subjectHuman urine samplespt_PT
dc.subjecticroextraction by packed sorbent (MEPS)pt_PT
dc.subjectUltra high performance liquid chromatographypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleAn improved analytical strategy combining microextraction by packed sorbent combined with ultra high pressure liquid chromatography for the determination of fluoxetine, clomipramine and their active metabolites in human urinept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage40pt_PT
oaire.citation.startPage30pt_PT
oaire.citation.titleJournal of Chromatography Apt_PT
oaire.citation.volume1408pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAlves
person.familyNameGonçalves
person.familyNameCâmara
person.givenNameVera
person.givenNameJoão
person.givenNameJosé
person.identifierG-3003-2013
person.identifier.ciencia-idE515-0AFA-CB1D
person.identifier.ciencia-id8C14-3188-9E44
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-1485-6032
person.identifier.orcid0000-0002-1265-6686
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridC-1348-2019
person.identifier.scopus-author-id55792994000
person.identifier.scopus-author-id52163735200
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication7971c475-3f08-4462-96b1-79e96c9615a4
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
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