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Evaluation of fucoxanthin contents in seaweed biomass by vortex-assisted solid-liquid microextraction using high-performance liquid chromatography with photodiode array detection

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dc.contributor.authorNunes, N.
dc.contributor.authorLeça, João M.
dc.contributor.authorPereira, Ana C.
dc.contributor.authorPereira, Vanda
dc.contributor.authorFerraz, S.
dc.contributor.authorBarreto, Maria Carmo
dc.contributor.authorMarques, José C.
dc.contributor.authorCarvalho, M.A.A. Pinheiro de
dc.date.accessioned2021-03-08T11:14:59Z
dc.date.available2021-03-08T11:14:59Z
dc.date.issued2019
dc.description.abstractFucoxanthin is considered an important marine bioactive compound with biological properties with promising effects, namely on health. A simple and efficient analytical methodology is proposed for its quantification in seaweed biomass by using vortex-assisted solid-liquid microextraction (VASLME) followed by reversed phase high-performance liquid chromatography (RP-HPLC) photodiode array detection (PDA) analysis. This microextraction uses reduced quantities of sample (25 mg) and solvent (300 μL of ethanol) to efficiently extract this high-valued xanthophyll, in a vortex time of 15 min. These extraction parameters were optimized performing a Central Composite Design (CCD) analysis, running 32 individual experiments. In turn, the method validation was assessed. The linearity of the method was confirmed (R2 = 0.99998) in a concentration range from 12 to 3600 μg·g−1 dw. Also, good sensitivity and accuracy results were observed through the LOD (3.33 μg·g−1 ), LOQ (10.09 μg·g−1 ) and recovery (varied from 95 to 97%) assessments. Good precision was also verified, with intra-day variation within 2.0–3.3%, and inter-day within 1.0–3.8%. Matrix effect was also evaluated and an acceptable variation of 3.4% was found. The method applicability was confirmed by the analysis of 22 seaweed biomass samples and fucoxanthin content was found to vary from about 10 to 853 μg·g−1 dw. This method demonstrated a good performance and can be successfully implemented for a rapid, reliable and accurate screening of fucoxanthin in seaweed biomass.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNunes, N., Leça, J. M., Pereira, A. C., Pereira, V., Ferraz, S., Barreto, M. C., ... & de Carvalho, M. P. (2019). Evaluation of fucoxanthin contents in seaweed biomass by vortex-assisted solid-liquid microextraction using high-performance liquid chromatography with photodiode array detection. Algal Research, 42, 101603. https://doi.org/10.1016/j.algal.2019.101603pt_PT
dc.identifier.doi10.1016/j.algal.2019.101603pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3155
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectFucoxanthinpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleEvaluation of fucoxanthin contents in seaweed biomass by vortex-assisted solid-liquid microextraction using high-performance liquid chromatography with photodiode array detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUID/BIA/00329/2013
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIA%2F00329%2F2013/PT
oaire.citation.startPage101603pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume42pt_PT
oaire.fundingStream5876
person.familyNameNunes
person.familyNameLeça
person.familyNamePereira
person.familyNamePereira
person.familyNameFerraz Alves
person.familyNameRoque Lino Felgueiras Barreto
person.familyNameMarques
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameNuno
person.givenNameJoão Micael
person.givenNameAna Cristina
person.givenNameVanda
person.givenNameSónia
person.givenNameMaria do Carmo
person.givenNameJosé C.
person.givenNameMiguel Angelo
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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