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Evaluation of fucoxanthin contents in seaweed biomass by vortex-assisted solid-liquid microextraction using high-performance liquid chromatography with photodiode array detection

2019Journal article Open access
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dc.contributor.authorNunes, N.
dc.contributor.authorLeça, João M.
dc.contributor.authorPereira, Ana C.
dc.contributor.authorPereira, Vanda
dc.contributor.authorFerraz, S.
dc.contributor.authorBarreto, Maria Carmo
dc.contributor.authorMarques, José C.
dc.contributor.authorCarvalho, M.A.A. Pinheiro de
dc.date.accessioned2021-03-08T11:14:59Z
dc.date.available2021-03-08T11:14:59Z
dc.date.issued2019
dc.description.abstractFucoxanthin is considered an important marine bioactive compound with biological properties with promising effects, namely on health. A simple and efficient analytical methodology is proposed for its quantification in seaweed biomass by using vortex-assisted solid-liquid microextraction (VASLME) followed by reversed phase high-performance liquid chromatography (RP-HPLC) photodiode array detection (PDA) analysis. This microextraction uses reduced quantities of sample (25 mg) and solvent (300 μL of ethanol) to efficiently extract this high-valued xanthophyll, in a vortex time of 15 min. These extraction parameters were optimized performing a Central Composite Design (CCD) analysis, running 32 individual experiments. In turn, the method validation was assessed. The linearity of the method was confirmed (R2 = 0.99998) in a concentration range from 12 to 3600 μg·g−1 dw. Also, good sensitivity and accuracy results were observed through the LOD (3.33 μg·g−1 ), LOQ (10.09 μg·g−1 ) and recovery (varied from 95 to 97%) assessments. Good precision was also verified, with intra-day variation within 2.0–3.3%, and inter-day within 1.0–3.8%. Matrix effect was also evaluated and an acceptable variation of 3.4% was found. The method applicability was confirmed by the analysis of 22 seaweed biomass samples and fucoxanthin content was found to vary from about 10 to 853 μg·g−1 dw. This method demonstrated a good performance and can be successfully implemented for a rapid, reliable and accurate screening of fucoxanthin in seaweed biomass.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNunes, N., Leça, J. M., Pereira, A. C., Pereira, V., Ferraz, S., Barreto, M. C., ... & de Carvalho, M. P. (2019). Evaluation of fucoxanthin contents in seaweed biomass by vortex-assisted solid-liquid microextraction using high-performance liquid chromatography with photodiode array detection. Algal Research, 42, 101603. https://doi.org/10.1016/j.algal.2019.101603pt_PT
dc.identifier.doi10.1016/j.algal.2019.101603pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3155
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectFucoxanthinpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleEvaluation of fucoxanthin contents in seaweed biomass by vortex-assisted solid-liquid microextraction using high-performance liquid chromatography with photodiode array detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIA%2F00329%2F2013/PT
oaire.citation.startPage101603pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume42pt_PT
oaire.fundingStream5876
person.familyNameNunes
person.familyNameLeça
person.familyNamePereira
person.familyNamePereira
person.familyNameFerraz Alves
person.familyNameRoque Lino Felgueiras Barreto
person.familyNameMarques
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameNuno
person.givenNameJoão Micael
person.givenNameAna Cristina
person.givenNameVanda
person.givenNameSónia
person.givenNameMaria do Carmo
person.givenNameJose C
person.givenNameMiguel Angelo
person.identifier1083569
person.identifier41146
person.identifierN-8704-2015
person.identifier1218558
person.identifier.ciencia-idD412-34DB-BB84
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person.identifier.orcid0000-0001-7939-1371
person.identifier.orcid0000-0001-5797-8913
person.identifier.orcid0000-0002-4832-4341
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridA-7681-2015
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person.identifier.scopus-author-id7203033313
person.identifier.scopus-author-id8758577600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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