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The effect of PAMAM dendrimers on mesenchymal stem cell viability and differentiation

2012Journal article Restricted access
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dc.contributor.authorGonçalves, M.
dc.contributor.authorCastro, R.
dc.contributor.authorRodrigues, J.
dc.contributor.authorTomás, H.
dc.date.accessioned2019-06-21T08:48:56Z
dc.date.available2019-06-21T08:48:56Z
dc.date.issued2012
dc.description.abstractStem cells and nanomaterials are two new and exciting fields of science that are evolving very fast and that are starting to establish ties. Nanomaterials should, however, be designed to interact with stem cells without compromising their biological characteristics, in other words, without affecting their viability and differentiation potential. In the present report and for the first time, the effects of poly(amidoamine) (PAMAM) dendrimers on the viability and differentiation ability towards the osteogenic and adipogenic lineages of human mesenchymal stem cells (hMSCs) are systematically evaluated. Studies were done as a function of the cell culture media composition and PAMAM dendrimer surface functionalization, generation, and concentration. hMSCs were exposed to amino and hydroxyl (generations 2, 4 and 6), and carboxylate (generations 1.5, 3.5 and 5.5) functionalized dendrimers, at two different concentrations (10 μg/mL and 0.5 μg/mL), for a period of 21 days. Overall, the results revealed that amino functionalized dendrimers can be severely cytotoxic, the extension of cell death being dependent on the concentration of amino groups in solution. However, in all cases, the differentiation of hMSCs towards the osteogenic and adipogenic phenotypes seems not to be affected as demonstrated by staining in in vitro cultures.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGoncalves, M., Castro, R., Rodrigues, J., & Tomas, H. (2012). The effect of PAMAM dendrimers on mesenchymal stem cell viability and differentiation. Current medicinal chemistry, 19(29), 4969-4975.pt_PT
dc.identifier.issn0929-8673
dc.identifier.urihttp://hdl.handle.net/10400.13/2413
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBentham Science Publisherspt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectAdipogenesispt_PT
dc.subjectPAMAMpt_PT
dc.subjectAlkaline phosphatase activitypt_PT
dc.subjectCell viabilitypt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectDendrimerpt_PT
dc.subjectDifferentiationpt_PT
dc.subjectHuman mesenchymal stem cellspt_PT
dc.subjectOsteogenesispt_PT
dc.subjectPoly(amidoamine)pt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleThe effect of PAMAM dendrimers on mesenchymal stem cell viability and differentiationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage4975pt_PT
oaire.citation.startPage4969pt_PT
oaire.citation.titleCurrent Medicinal Chemistrypt_PT
oaire.citation.volume19(29)pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGonçalves
person.familyNameCastro
person.familyNameRodrigues
person.familyNameTomás
person.givenNameMara
person.givenNameRita
person.givenNameJoão
person.givenNameHelena
person.identifier556975
person.identifier.ciencia-id6010-1863-4B11
person.identifier.ciencia-id8314-881E-4986
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0002-0332-3548
person.identifier.orcid0000-0002-9337-062X
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridA-2497-2013
person.identifier.ridB-6816-2008
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id55509603000
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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