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Quantification of polyphenols with potential antioxidant properties in wines using reverse phase HPLC

2008-02Journal article Open access
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dc.contributor.authorPaixão, Neuza
dc.contributor.authorPereira, Vanda Nulita Gomes
dc.contributor.authorMarques, José C.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2015-12-15T11:32:26Z
dc.date.available2015-12-15T11:32:26Z
dc.date.issued2008-02
dc.description.abstractA RP-HPLC method with photodiode array detection (DAD) was developed to separate, identify and quantify simultaneously the most representative phenolic compounds present in Madeira and Canary Islands wines. The optimized chromatographic method was carefully validated in terms of linearity, precision, accuracy and sensitivity. A high repeatability and a good stability of phenolics retention times (a3%) were obtained, as well as relative peak area. Also high recoveries were achieved, over 80.3%. Polyphenols calibration curves showed a good linearity (r2 A0.994) within test ranges. Detection limits ranged between 0.03 and 11.5 lg/mL for the different polyphenols. A good repeatability was obtained, with intra-day variations less than 7.9%. The described method was successfully applied to quantify several polyphenols in 26 samples of different kinds of wine (red, ros and white wines) from Madeira and Canary Islands. Gallic acid was by far the most predominant acid. It represents more than 65% of all phenolics, followed by p-coumaric and caffeic acids. The major flavonoid found in Madeira wines was trans-resveratrol. In some wines, (–)-epicatechin was also found in highest amount. Canary wines were shown to be rich in gallic, caffeic and p-coumaric acids and quercetin.pt_PT
dc.identifier.citationPaixão, N., Pereira, V., Marques, J. C., & Câmara, J. S. (2008). Quantification of polyphenols with potential antioxidant properties in wines using reverse phase HPLC. Journal of separation science, 31(12), 2189-2198.pt_PT
dc.identifier.doi10.1002/jssc.200800021pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/958
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWILEY-VCHVerlagGmbHpt_PT
dc.subjectAntioxidantspt_PT
dc.subjectHPLC-photodiodearraydetectionpt_PT
dc.subjectPolyphenolspt_PT
dc.subjectWinespt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleQuantification of polyphenols with potential antioxidant properties in wines using reverse phase HPLCpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2198pt_PT
oaire.citation.startPage2189pt_PT
oaire.citation.titleJournal of separation sciencept_PT
oaire.citation.volume31(12)pt_PT
person.familyNamePereira
person.familyNameMarques
person.familyNameCâmara
person.givenNameVanda
person.givenNameJose C
person.givenNameJosé
person.identifier41146
person.identifierG-3003-2013
person.identifier.ciencia-id0115-071E-87E8
person.identifier.ciencia-id1713-6DA1-A551
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0003-0772-8953
person.identifier.orcid0000-0002-4832-4341
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridJ-8697-2013
person.identifier.ridF-3014-2010
person.identifier.scopus-author-id36857887700
person.identifier.scopus-author-id7203033313
person.identifier.scopus-author-id10140393000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationac913112-adb8-4fe0-88b0-f49e6fb5635d
relation.isAuthorOfPublication08f4685f-b917-410a-b153-59911ff7d75e
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscoveryac913112-adb8-4fe0-88b0-f49e6fb5635d

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