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High-value lipids accumulation by Pavlova pinguis as a response to nitrogen-induced changes

2022Journal article Open access
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dc.contributor.authorFernandes, Tomásia
dc.contributor.authorCordeiro, Nereida
dc.date.accessioned2022-02-10T14:18:52Z
dc.date.available2022-02-10T14:18:52Z
dc.date.issued2022
dc.description.abstractThe challenges of exploring the potential of microalgal strains for biotechnological applications include the optimization of their cell growth and chemical composition. To overcome this, it is essential to understand the mechanisms that lead to the accumulation of desired products within microalgal cells. In this study, a gradient of nitrogen as nitrate (NO−3 − N) concentrations was used for the preparation of P. pinguis growth medium. The algal growth dynamics, pigments, nutrient uptake, and detailed lipid composition across treatments were assessed. Increasing the NO− 3 − N level led to higher lipid content (21%), a higher cell uptake rate (0.20 pg NO− 3 − N cell− 1 d− 1 ) and more accumulation of chlorophylls, carotenoids, and high-value lipids. Eicosapentaenoic acid, essential fatty acids, phytol and stigmasterol were the key high-value lipids that were positively influenced by higher NO− 3 − N levels. High NO− 3 − N conditions induced an increase of 54% in total sterol content, while low NO− 3 − N conditions resulted in increased proportions of saturated fatty acids (66% more) and decreased proportions of polyunsaturated fatty acids (14% less). The low NO− 3 − N level also led to higher amounts of monoglyceride (1.60 mg g− 1 ; 64% more). The analysis of P. pinguis lipids before and after hydrolysis provided an insight into the composition of the esterified lipids across treatments. NO− 3 − N supplementation was revealed to be an effective strategy for enhancing P. pinguis lipid composition, for nutraceutical and pharmaceutical industries (high NO− 3 − N level). A greater understanding of the NO− 3 − N uptake and the use efficiency by P. pinguis was reached, showing its potential for further biotechnological applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, T., & Cordeiro, N. (2022). High-value lipids accumulation by Pavlova pinguis as a response to nitrogen-induced changes. Biomass and Bioenergy, 158, 106341.pt_PT
dc.identifier.doi10.1016/j.biombioe.2022.106341pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4075
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPavlova pinguispt_PT
dc.subjectNO− 3 − Nsupplypt_PT
dc.subjectHigh-value lipidspt_PT
dc.subjectBiotechnological applicationpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleHigh-value lipids accumulation by Pavlova pinguis as a response to nitrogen-induced changespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT
oaire.citation.startPage106341pt_PT
oaire.citation.titleBiomass and Bioenergypt_PT
oaire.citation.volume158pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGomez Fernandes
person.familyNamecordeiro
person.givenNameTomásia Micaela
person.givenNamenereida
person.identifier1612636
person.identifier.ciencia-id521A-0F62-EA39
person.identifier.orcid0000-0002-6157-4177
person.identifier.orcid0000-0001-6006-3415
person.identifier.ridO-2253-2019
person.identifier.scopus-author-id7004319456
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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