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Evaluation of wheat germplasm from the Madeira and Canary archipelagos using a single molecular marker. A rapid screening method for identification of durum wheat accessions

2007Journal article Open access
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dc.contributor.authorAndrade, V.
dc.contributor.authorSantos, T.
dc.contributor.authorAfonso Morales, D.
dc.contributor.authorCosta, G.
dc.contributor.authorCarvalho, M. Pinheiro de
dc.date.accessioned2021-03-18T12:20:46Z
dc.date.available2021-03-18T12:20:46Z
dc.date.issued2007
dc.description.abstractThis work aims to present a rapid and precise screening method of wheat germplasm and identification of durum wheat accessions in germplasm collections. Fifty-two wheat accessions maintained in the ISOPlexis germplasm bank at the Madeira University, Portugal, and 72 accessions from the Centre for Conservation of Crop Biodiversity of Tenerife (CCBAT), Spain, have been screened for their specific ploidy status using the molecular marker Dgas44. We have demonstrated that the Dgas44 sequence is effective in the screening of Madeiran and Canarian wheat accessions. This screening method permitted the detection of 10 and 11 durum accessions among Madeiran and Canarian wheats, accounting for 19.2 and 15.3% of screened collections, respectively. The obtained results have shown a 100% of cases correspondence with the previously performed morphological identification of the Madeiran wheats. It also permitted rectification and clarification of previous classification of some accessions based only on the morphological traits. The PCR based assay was directly applicable to the screening of seeds and was suitable for detecting seed mixtures in accessions. This rapid method has been proven to be a useful tool in gene bank accessions management including verification of their ploidy status and detection of seed lots adulteration.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAndrade, V., Santos, T., Afonso Morales, D., Costa, G., & Carvalho, M. P. (2007). Evaluation of wheat germplasm from the Madeira and Canary archipelagos using a single molecular marker. A rapid screening method for identification of durum wheat accessions. Cereal Research Communications,35(3), 1397-1404. DOI: 10.1556/CRC.35.2007.3.4pt_PT
dc.identifier.doi10.1556/CRC.35.2007.3.4pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3179
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAkadémiai Kiadópt_PT
dc.relationMolecular approaches in the identification local crop varieties and evaluation of their seed quality
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDurum wheatpt_PT
dc.subjectGermplasmpt_PT
dc.subjectD genomept_PT
dc.subjectDNApt_PT
dc.subjectPCRpt_PT
dc.subjectDgas44pt_PT
dc.subjectMadeira Archipelago (Portugal)pt_PT
dc.subjectCanary Archipelago (Spain)pt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleEvaluation of wheat germplasm from the Madeira and Canary archipelagos using a single molecular marker. A rapid screening method for identification of durum wheat accessionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular approaches in the identification local crop varieties and evaluation of their seed quality
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FAGR%2F56188%2F2004/PT
oaire.citation.endPage1404pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage1397pt_PT
oaire.citation.titleCereal Research Communicationspt_PT
oaire.citation.volume35pt_PT
oaire.fundingStreamPOCI
person.familyNamePereira da Costa
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameGraça Maria
person.givenNameMiguel Angelo
person.identifier1218558
person.identifier.ciencia-id4B1C-4144-D839
person.identifier.ciencia-id4610-6741-6816
person.identifier.orcid0000-0003-0277-3376
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridAEE-0913-2022
person.identifier.scopus-author-id8758577600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication2e433c48-88f8-40a2-b21f-de78b44aab1c
relation.isAuthorOfPublication.latestForDiscovery2e433c48-88f8-40a2-b21f-de78b44aab1c
relation.isProjectOfPublication757db207-3099-4f2a-9911-608b70a4901a
relation.isProjectOfPublication.latestForDiscovery757db207-3099-4f2a-9911-608b70a4901a

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