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Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors

2009Journal article Restricted access
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dc.contributor.authorSantos, José Luís
dc.contributor.authorOramas, Elena
dc.contributor.authorPêgo, Ana Paula
dc.contributor.authorGranja, Pedro Lopes
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-07-09T09:28:18Z
dc.date.available2019-07-09T09:28:18Z
dc.date.issued2009
dc.description.abstractThis paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a beta-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing plasmid were used. All quantitative (alkaline phosphatase activity, osteocalcin secretion and calcium deposition) and qualitative (von Kossa staining) osteogenic markers were significantly stronger in transfected cells when compared to non-transfected ones. This study not only clearly demonstrates that a low transfection level can be sufficient for inducing in vitro differentiation of MSCs to the osteoblast phenotype but also highlights the importance of focusing research on the development of gene delivery vectors in the concrete application.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSantos, J. L., Oramas, E., Pêgo, A. P., Granja, P. L., & Tomás, H. (2009). Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors. Journal of Controlled Release, 134(2), 141-148.pt_PT
dc.identifier.doi10.1016/j.jconrel.2008.11.007pt_PT
dc.identifier.issn0168-3659
dc.identifier.urihttp://hdl.handle.net/10400.13/2464
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectPAMAM dendrimerspt_PT
dc.subjectBone regenerationpt_PT
dc.subjectNonviral gene deliverypt_PT
dc.subjectMesenchymal stem cellspt_PT
dc.subjectBone morphogenetic proteinpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleOsteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectorspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F19450%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BEB%2F71161%2F2006/PT
oaire.citation.endPage148pt_PT
oaire.citation.startPage141pt_PT
oaire.citation.titleJournal of Controlled Releasept_PT
oaire.citation.volume134(2)pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
person.familyNamePêgo
person.familyNameTomás
person.givenNameAna Paula
person.givenNameHelena
person.identifier164495
person.identifier556975
person.identifier.ciencia-id6114-92F0-E64C
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0001-5169-328X
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridC-6942-2012
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id56053889300
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
relation.isAuthorOfPublication.latestForDiscovery905a2f89-0018-47d3-850d-4b09bed65b5f
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