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Osteoblastic behavior of human bone marrow cells cultured over adsorbed collagen layer, over surface of collagen gels, and inside collagen gels

2009Journal article Restricted access
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dc.contributor.authorFernandes, Luís F.
dc.contributor.authorCosta, Maria A.
dc.contributor.authorFernandes, Maria H.
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-07-09T10:28:21Z
dc.date.available2019-07-09T10:28:21Z
dc.date.issued2009
dc.description.abstractWhile collagen type I is often used as a substrate for cell culturing and as a coating in biomedical implants, as far as we know a simple systematic study comparing the effects of the different presentations of collagen type I on the osteoblastic behavior of cells is missing. In this work, human bone marrow cells (hBMCs) were cultured under osteoblastic-inducing conditions, for 21 days, over a layer of adsorbed collagen (monomeric) and on the surface and inside collagen gels (fibrillar). Comparison was made based on three classical parameters; cell proliferation/viability, alkaline phosphatase (ALP) activity, and production of mineral deposits. The three types of collagen type I substrates allowed the adhesion, proliferation, and the osteoblastic differentiation of cells. However, hBMCs behavior was influenced by the monomeric/fibrillar and 2-/3-dimensional nature of the collagen substrates, namely: monomeric collagen favored cell attachment; cells on 2D substrates presented higher proliferation rates during the exponential phase of growth with formation of spiral-like multilayered structures; cells seeded inside 3D collagen gels formed a regular dense cellular mesh and had a low proliferating rate; cells cultured over or inside fibrillar collagen differentiated faster, with the 3D cultures presenting higher levels of ALP activity; and the extension of mineralization was greater for the cultures done over or inside fibrillar collagen. Thus, cells cultured over collagen gels showed both the ability for cell proliferation and for earlier differentiation, a fact that can be exploited in the biomaterials field.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, L. F., Costa, M. A., Fernandes, M. H., & Tomás, H. (2009). Osteoblastic behavior of human bone marrow cells cultured over adsorbed collagen layer, over surface of collagen gels, and inside collagen gels. Connective tissue research, 50(5), 336-346.pt_PT
dc.identifier.doi10.1080/03008200902855909pt_PT
dc.identifier.issn0300-8207
dc.identifier.urihttp://hdl.handle.net/10400.13/2465
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relationThree-dimensional systems of collagen type I, proteoglycans and growth factors for bone cell proliferation and differentiation
dc.subjectCollagen type Ipt_PT
dc.subjectOsteogenesispt_PT
dc.subjectHuman bone marrow cellspt_PT
dc.subjectCell differentiationpt_PT
dc.subjectBiomaterialspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleOsteoblastic behavior of human bone marrow cells cultured over adsorbed collagen layer, over surface of collagen gels, and inside collagen gelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThree-dimensional systems of collagen type I, proteoglycans and growth factors for bone cell proliferation and differentiation
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FFCB%2F33620%2F2000/PT
oaire.citation.endPage346pt_PT
oaire.citation.startPage336pt_PT
oaire.citation.titleConnective Tissue Researchpt_PT
oaire.citation.volume50(5)pt_PT
oaire.fundingStreamPOCI
person.familyNameTomás
person.givenNameHelena
person.identifier556975
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
relation.isAuthorOfPublication.latestForDiscovery3a747114-6ab9-454b-985b-00cbf792e273
relation.isProjectOfPublication1a0d4ba5-f74c-4ea6-80c3-c2cf14410612
relation.isProjectOfPublication.latestForDiscovery1a0d4ba5-f74c-4ea6-80c3-c2cf14410612

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