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Proļ¬ling allergic asthma volatile metabolic patterns using a headspace-solid phase microextraction/gas chromatography based methodology

2011-06Journal article Open access
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dc.contributor.authorCaldeira, M.
dc.contributor.authorBarros, AntĆ³nio S.
dc.contributor.authorBilelo, M. J.
dc.contributor.authorParada, A.
dc.contributor.authorCĆ¢mara, J. S.
dc.contributor.authorRocha, SĆ­lvia M.
dc.date.accessioned2015-12-09T10:10:42Z
dc.date.available2015-12-09T10:10:42Z
dc.date.issued2011-06
dc.description.abstractAllergicasthmarepresentsanimportantpublichealthissuewithsigniļ¬cantgrowthovertheyears,especially in the paediatric population. Exhaled breath is a non-invasive, easily performed and rapid method forobtainingsamplesfromthelowerrespiratorytract.Inthepresentmanuscript,themetabolicvolatile proļ¬les of allergic asthma and control children were evaluated by headspace solid-phase microextraction combined with gas chromatographyā€“quadrupole mass spectrometry (HS-SPME/GCā€“qMS). The lack ofstudiesinbreathofallergicasthmaticchildrenbyHS-SPMEledtothedevelopmentofanexperimental design to optimize SPME parameters. To fulļ¬l this objective, three important HS-SPME experimental parameters that inļ¬‚uence the extraction efļ¬ciency, namely ļ¬bre coating, temperature and time extractions were considered. The selected conditions that promoted higher extraction efļ¬ciency corresponding to the higher GC peak areas and number of compounds were: DVB/CAR/PDMS coating ļ¬bre, 22ā—¦C and 60min as the extraction temperature and time, respectively. The suitability of two containers, 1L TedlarĀ® bags and BIOVOCĀ®, for breath collection and intra-individual variability were also investigated. The developed methodology was then applied to the analysis of children exhaled breath with allergicasthma(35),fromwhich13hadalsoallergicrhinitis,andhealthycontrolchildren(15),allowing to identify 44 volatiles distributed over the chemical families of alkanes (linear and ramiļ¬ed) ketones, aromatic hydrocarbons, aldehydes, acids, among others. Multivariate studies were performed by Partial LeastSquaresā€“DiscriminantAnalysis(PLSā€“DA)usingasetof28selectedmetabolitesanddiscrimination between allergic asthma and control children was attained with a classiļ¬cation rate of 88%. The allergic asthma paediatric population was characterized mainly by the compounds linked to oxidative stress, such as alkanes and aldehydes. Furthermore, more detailed information was achieved combining the volatile metabolic data, suggested by PLSā€“DA model, and clinical data.pt_PT
dc.identifier.citationCaldeira, M., Barros, A. S., Bilelo, M. J., Parada, A., Camara, J. S., & Rocha, S. M. (2011). Profiling allergic asthma volatile metabolic patterns using a headspace-solid phase microextraction/gas chromatography based methodology. Journal of Chromatography A, 1218(24), 3771-3780.pt_PT
dc.identifier.doi10.1016/j.chroma.2011.04.026pt_PT
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10400.13/938
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationDESENVOLV. DE UMA METODOLOGIA PARA A DETECƇƃO DE BIOMARCADORES DE DOENƇAS RESPIRATƓRIAS ALƉRGICAS BASEADA NA APLICAƇƃO DA MICROEXTRACƇƃO EM FASE SƓLIDA ACOPLADA A CROMATOGRAFIA EM FASE GASOSA. CONTRIBUTOS PARA A DEFINIƇƃO DE UM MEIO DIAGNƓSTICO
dc.subjectAllergic asthmapt_PT
dc.subjectExhaled breathpt_PT
dc.subjectVolatile metabolitespt_PT
dc.subjectHeadspace solid-phase microextractionpt_PT
dc.subjectGas chromatography-mass spectrometrypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de CiĆŖncias Exatas e da Engenhariapt_PT
dc.subjectCentro de QuĆ­mica da Madeira
dc.titleProļ¬ling allergic asthma volatile metabolic patterns using a headspace-solid phase microextraction/gas chromatography based methodologypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDESENVOLV. DE UMA METODOLOGIA PARA A DETECƇƃO DE BIOMARCADORES DE DOENƇAS RESPIRATƓRIAS ALƉRGICAS BASEADA NA APLICAƇƃO DA MICROEXTRACƇƃO EM FASE SƓLIDA ACOPLADA A CROMATOGRAFIA EM FASE GASOSA. CONTRIBUTOS PARA A DEFINIƇƃO DE UM MEIO DIAGNƓSTICO
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PIDDAC/SFRH%2FBD%2F40374%2F2007/PT
oaire.citation.endPage3780pt_PT
oaire.citation.startPage3771pt_PT
oaire.citation.titleJournal of Chromatography Apt_PT
oaire.citation.volume1218(24)pt_PT
oaire.fundingStreamPIDDAC
person.familyNameCaldeira
person.familyNameBarros
person.familyNameCĆ¢mara
person.familyNameRocha
person.givenNameMichael
person.givenNameAntĆ³nio
person.givenNameJosƩ
person.givenNameSĆ­lvia M.
person.identifierR-000-200
person.identifierG-3003-2013
person.identifier.ciencia-id4714-1135-72CC
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.ciencia-id7B1E-AE7B-1100
person.identifier.orcid0000-0001-5371-7281
person.identifier.orcid0000-0002-9103-5852
person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0002-0396-3019
person.identifier.ridA-2461-2013
person.identifier.scopus-author-id16300962200
person.identifier.scopus-author-id56130256800
person.identifier.scopus-author-id10140393000
person.identifier.scopus-author-id7006653213
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationce73ce9c-932a-404b-93fe-cfefd5181554
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relation.isProjectOfPublication54ce93bf-6971-40a4-8a8c-615abc0d3878
relation.isProjectOfPublication.latestForDiscovery54ce93bf-6971-40a4-8a8c-615abc0d3878

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