Repository logo
 
Publication

Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer technique

2016Journal article Open access
Simple item page
dc.contributor.authorRamalingam, Kirthiga
dc.contributor.authorCastro, Rita
dc.contributor.authorPires, Pedro
dc.contributor.authorShi, Xiangyang
dc.contributor.authorRodrigues, João
dc.contributor.authorXiao, Shili
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-06-25T09:39:23Z
dc.date.available2019-06-25T09:39:23Z
dc.date.issued2016
dc.description.abstractA gene delivery platform for potential use in tissue engineering applications was developed by surface functionalization of biodegradable electrospun poly(lactic-co-glycolic acid) (PLGA) fibers with nanolayers of chitosan (cationic polymer) and alginate (anionic polymer) using the Layer-by-Layer (LbL) technique. The developed system not only supported the attachment and growth of human Mesenchymal Stem Cells (hMSCs), but also was capable of delivering pDNA/dendrimer complexes and inducing cell differentiation towards the osteogenic lineage when a pDNA codifying for human Bone Morphogenetic Protein-2 (BMP-2) was used. Beyond providing a means for pDNA/dendrimer complex immobilization, the polyelectrolyte coating conferred sustained release properties to the scaffold that resulted in pDNA protection from degradation. The polyelectrolyte coating, by itself, also contributed to enhance cell differentiation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRamalingam, K., Castro, R., Pires, P., Shi, X., Rodrigues, J., Xiao, S., & Tomás, H. (2016). Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer technique. RSC Advances, 6(99), 97116-97128.pt_PT
dc.identifier.doi10.1039/C6RA22444Jpt_PT
dc.identifier.issn2046-2069
dc.identifier.urihttp://hdl.handle.net/10400.13/2427
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationNEW MATERIALS FOR BIOMEDICAL APPLICATIONS OBTAINED BY THE SELF-ASSEMBLY OF DENDRIMER-SINGLE STRANDED DNA CONJUGATES
dc.relationMadeira Chemistry Research Centre
dc.subjectGene delivery using dendrimer/pDNA complexespt_PT
dc.subjectElectrospun fiberspt_PT
dc.subjectLayer-by-layer techniquept_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleGene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer techniquept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNEW MATERIALS FOR BIOMEDICAL APPLICATIONS OBTAINED BY THE SELF-ASSEMBLY OF DENDRIMER-SINGLE STRANDED DNA CONJUGATES
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F75420%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00674%2F2013/PT
oaire.citation.endPage97128pt_PT
oaire.citation.startPage97116pt_PT
oaire.citation.titleRSC Advancespt_PT
oaire.citation.volume6(99)pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCastro
person.familyNameDuarte Louzeiro Pires
person.familyNameShi
person.familyNameRodrigues
person.familyNameXiao
person.familyNameTomás
person.givenNameRita
person.givenNamePedro Filipe
person.givenNameXiangyang
person.givenNameJoão
person.givenNameShili
person.givenNameHelena
person.identifierC-3387-2012
person.identifier556975
person.identifier.ciencia-id8314-881E-4986
person.identifier.ciencia-idD413-1517-1263
person.identifier.ciencia-idBA11-1437-B948
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0002-9337-062X
person.identifier.orcid0000-0001-8583-7990
person.identifier.orcid0000-0001-6785-6645
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0001-9955-2064
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridA-2497-2013
person.identifier.ridA-1289-2007
person.identifier.ridB-6816-2008
person.identifier.ridA-3537-2013
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id7003655474
person.identifier.scopus-author-id7402953116
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id35192467200
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationceb211d0-5522-42ba-bbf0-adfc23f37f84
relation.isAuthorOfPublicationcd8a2ad2-3636-4a25-a806-472fa59e9d1e
relation.isAuthorOfPublicationc6b4b8c5-1241-4906-b4b9-ace5576b0b62
relation.isAuthorOfPublication53031fca-b2e7-4ce4-bda9-61e486da4547
relation.isAuthorOfPublication7cd406f1-c9be-416f-8fe5-7e2120a47645
relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
relation.isAuthorOfPublication.latestForDiscoveryceb211d0-5522-42ba-bbf0-adfc23f37f84
relation.isProjectOfPublication32705d0f-e122-40bc-9093-a14f889d087a
relation.isProjectOfPublicationfd9571e0-489f-4866-8f87-99dadd8a09d5
relation.isProjectOfPublication.latestForDiscovery32705d0f-e122-40bc-9093-a14f889d087a

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Gene delivery using dendrimer pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer techniqu.pdf
Size:
1.3 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artigos em revistas internacionais