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Quantification of artemisinin in Artemisia annua extracts by 1H‐NMR

2008Journal article Open access
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dc.contributor.authorCastilho, Paula C.
dc.contributor.authorGouveia, Sandra C.
dc.contributor.authorRodrigues, Ana I.
dc.date.accessioned2021-04-08T14:41:14Z
dc.date.available2021-04-08T14:41:14Z
dc.date.issued2008
dc.description.abstractArtemisinin is a polycyclic sesquiterpene lactone that is highly effective against multidrug-resistant strains of Plasmodium falciparum, the etiological agent of the most severe form of malaria. Determination of artemisinin in the source plant, Artemisia annua, is a challenging problem since the compound is present in very low concentrations, is thermolabile and unstable, and lacks chromophoric or fluorophoric groups. The ain of this study was to develop a simple protocol for the quantification of artemisinin in a plant extract using an (1)H-NMR method. Samples were prepared by extraction of leaf material with acetone, treatment with activated charcoal to remove chlorophylls and removal of solvent. (1)H-NMR spectra were measured on samples dissolved in deuterochloroform with tert-butanol as internal standard. Quantification was carried out using the using the delta 5.864 signal of artemisinin and the delta 1.276 signal of tert-butanol. The method was optimised and fully validated against a reference standard of artemisinin. The results were compared with those obtained from the same samples quantified using an HPLC-refractive index (RI) method. The (1)H-NMR method gave a linear response for artemisinin within the range 9.85-97.99 mm (r(2) = 0.9968). Using the described method, yields of artemisinin in the range 0.77-1.06% were obtained from leaves of the A. annua hybrid CPQBA x POP, and these values were in agreement with those obtained using an HPLC-RI.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCastilho, P. C., Gouveia, S. C., & Rodrigues, A. I. (2008). Quantification of artemisinin in Artemisia annua extracts by 1H‐NMR. Phytochemical Analysis, 19(4), 329-334.pt_PT
dc.identifier.doi10.1002/pca.1053pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3263
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNMRpt_PT
dc.subjectQuantificationpt_PT
dc.subjectArtemisininpt_PT
dc.subjectArtemisia annuapt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleQuantification of artemisinin in Artemisia annua extracts by 1H‐NMRpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage334pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage329pt_PT
oaire.citation.titlePhytochemical Analysispt_PT
oaire.citation.volume19pt_PT
person.familyNameCastilho
person.familyNameGouveia-Figueira
person.givenNamePaula C
person.givenNameSandra
person.identifierA-4412-2013
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.orcid0000-0002-8303-4286
person.identifier.orcid0000-0002-8116-2794
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd84fd917-438e-43bf-ac6f-256562481c05
relation.isAuthorOfPublicationf17ed1c1-62a1-48be-8299-95e04f4a1c5c
relation.isAuthorOfPublication.latestForDiscoveryd84fd917-438e-43bf-ac6f-256562481c05

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