Repository logo
 
Publication

Extracellular volatilomic alterations induced by hypoxia in breast cancer cells

2020Journal article Open access
Simple item page
dc.contributor.authorTaware, Ravindra
dc.contributor.authorTaunk, Khushman
dc.contributor.authorKumar, Totakura V. S.
dc.contributor.authorPereira, Jorge A. M.
dc.contributor.authorCâmara, José S.
dc.contributor.authorNagarajaram, H. A.
dc.contributor.authorKundu, Gopal C.
dc.contributor.authorRapole, Srikanth
dc.date.accessioned2021-12-02T09:21:10Z
dc.date.available2021-12-02T09:21:10Z
dc.date.issued2020
dc.description.abstractIntroduction The metabolic shift induced by hypoxia in cancer cells has not been explored at volatilomic level so far. The volatile organic metabolites (VOMs) constitute an important part of the metabolome and their investigation could provide us crucial aspects of hypoxia driven metabolic reconfguration in cancer cells. Objective To identify the altered volatilomic response induced by hypoxia in metastatic/aggressive breast cancer (BC) cells. Methods BC cells were cultured under normoxic and hypoxic conditions and VOMs were extracted using HS-SPME approach and profled by standard GC–MS system. Univariate and multivariate statistical approaches (p<0.05, Log2 FC≥0.58/≤−0.58, PC1>0.13/<−0.13) were applied to select the VOMs diferentially altered after hypoxic treatment. Metabolic pathway analysis was also carried out in order to identify altered metabolic pathways induced by the hypoxia in the selected BC cells. Results Overall, 20 VOMs were found to be signifcantly altered (p<0.05, PC1>0.13/< −0.13) upon hypoxic exposure to BC cells. Further, cell line specifc volatilomic alterations were extracted by comparative metabolic analysis of aggressive (MDA-MB-231) vs. non-aggressive (MCF-7) cells incubated under hypoxia and normoxia. In this case, 15 and 12 VOMs each were found to be signifcantly altered in aggressive cells when exposed to hypoxic and normoxic condition respectively. Out of these, 9 VOMs were found to be uniquely associated with hypoxia, 6 were specifc to normoxia and 6 were found common to both the conditions. Formic acid was identifed as the most prominent molecule with higher abundance levels in aggressive as compared to non-aggressive cells in both conditions. Furthermore, metabolic pathway analyses revealed that fatty acid biosynthesis and nicotinate and nicotinamide metabolism were signifcantly altered in aggressive as compared to non-aggressive cells in normoxia and hypoxia respectively. Conclusions Higher formate overfow was observed in aggressive cells compared to non-aggressive cells incubated under both the conditions, reinforcing its correlation with aggressive and invasive cancer type. Moreover, under hypoxia, aggres sive cells preferred to be bioenergetically more efcient whereas, under normoxia, fatty acid biosynthesis was favoured when compared to non-aggressive cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTaware, R., Taunk, K., Kumar, T. V., Pereira, J. A., Câmara, J. S., Nagarajaram, H. A., ... & Rapole, S. (2020). Extracellular volatilomic alterations induced by hypoxia in breast cancer cells. Metabolomics, 16(2), 1-12. https://doi.org/10.1007/s11306-020-1635-xpt_PT
dc.identifier.doi10.1007/s11306-020-1635-xpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3877
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBreast cancer cellspt_PT
dc.subjectHypoxiapt_PT
dc.subjectExtracellular volatilomic alterationspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleExtracellular volatilomic alterations induced by hypoxia in breast cancer cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.citation.issue2pt_PT
oaire.citation.titleMetabolomicspt_PT
oaire.citation.volume16pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameTaware
person.familyNameTAUNK
person.familyNameAugusto Machado Pereira
person.familyNameCâmara
person.givenNameRavindra
person.givenNameKHUSHMAN
person.givenNameJorge
person.givenNameJosé
person.identifierG-3003-2013
person.identifier.ciencia-idEC12-EE8F-50E8
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0001-7893-2850
person.identifier.orcid0000-0003-2293-2936
person.identifier.orcid0000-0003-0316-5348
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id57195429801
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb98f82dc-4ae0-4abd-9ffe-ffa3149a56d2
relation.isAuthorOfPublication36807717-d306-40ab-949d-d6aab8f01c6e
relation.isAuthorOfPublication2f4d7adf-ab6b-40ab-85d5-73995a784bda
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscovery2f4d7adf-ab6b-40ab-85d5-73995a784bda
relation.isProjectOfPublication75aaadc4-cd17-4fec-ba9d-1d0e564b3731
relation.isProjectOfPublication.latestForDiscovery75aaadc4-cd17-4fec-ba9d-1d0e564b3731

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Extracellular volatilomic alterations induced by hypoxia.pdf
Size:
2.5 MB
Format:
Adobe Portable Document Format
Download

Collections

Artigos em revistas internacionais