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HPLC-DAD methodology for the quantification of organic acids, furans and polyphenols by direct injection of wine samples

2010-01Journal article Open access
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dc.contributor.authorPereira, Vanda Nulita Gomes
dc.contributor.authorCâmara, José S.
dc.contributor.authorCacho, Juan
dc.contributor.authorMarques, José C.
dc.date.accessioned2015-12-09T15:26:44Z
dc.date.available2015-12-09T15:26:44Z
dc.date.issued2010-01
dc.description.abstractThis article proposes a simple and sensitive HPLC method with photo-diode array detection for the analysis of organic acids, monomeric polyphenols and furanic compounds in wine samples by direct injection. The chromatographic separation of 8 organic acids, 2 furans and 22 phenolic compounds was carried out with a buffered solution (pH 2.70) and acetonitrile as mobile phases and a difunctionally bonded C18 stationary phase, Atlantis dC18 (250 4.6 mm, 5mm) column. The elution was performed in 12 min for the organic acids and in 60 min for the phenolic compounds, including phenolic acids, stilbenes and flavonoids. Target compounds were detected at 210 nm (organic acids, flavan-3-ols and benzoic acids), 254 nm (ellagic acid), 280 nm (furans and cinnamic acid), 315 nm (hydroxycinnamic acids and trans-resveratrol) and 360 nm (flavonoids). The RSD for the repeatability test (n55) of peak area and retention times were below 3.1 and 0.3%, respectively, for phenolics and below 1.0 and 0.2% for organic acids. The RSDs expressing the reproducibility of the method were higher than for the repeatability results but all below 9.0%. Method accuracy was evaluated by the recovery results, with averaged values between 80 and 104% for polyphenols and 97–105% for organic acids. The calibration curves, obtained by triplicate injection of standard solutions, showed good linearity with regression coefficients higher than 0.9982 for polyphenols and 0.9997 for organic acids. The LOD was in the range of 0.07–0.49 mg/L for polyphenols (cinnamic and gallic acids, respectively) and 0.001–0.046 g/L for organic acids (oxalic and lactic acids, respectively). The method was successfully used to measure and assess the polyphenolic fingerprint and organic acids profile of red, white, rose ´ and fortified wines.pt_PT
dc.identifier.citationPereira, V., Câmara, J. S., Cacho, J., & Marques, J. C. (2010). HPLC‐DAD methodology for the quantification of organic acids, furans and polyphenols by direct injection of wine samples. Journal of separation science, 33(9), 1204-1215.pt_PT
dc.identifier.doi10.1002/jssc.200900784pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/942
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley Intersciencept_PT
dc.relationO EFEITO DA ESTUFAGEM NA FORMAÇÃO DE COMPOSTOS AROMÁTICOS CARACTERÍSTICOS DO VINHO MADEIRA
dc.subjectDirect injectionpt_PT
dc.subjectFuranspt_PT
dc.subjectHPLC method with photo-diode array detectionpt_PT
dc.subjectOrganic acidspt_PT
dc.subjectPolyphenolspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleHPLC-DAD methodology for the quantification of organic acids, furans and polyphenols by direct injection of wine samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleO EFEITO DA ESTUFAGEM NA FORMAÇÃO DE COMPOSTOS AROMÁTICOS CARACTERÍSTICOS DO VINHO MADEIRA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI-2010/SFRH%2FBD%2F24177%2F2005/PT
oaire.citation.endPage1215pt_PT
oaire.citation.startPage1204pt_PT
oaire.citation.titleJournal of separation sciencept_PT
oaire.citation.volume33(9)pt_PT
oaire.fundingStreamPOCI-2010
person.familyNamePereira
person.familyNameCâmara
person.familyNameMarques
person.givenNameVanda
person.givenNameJosé
person.givenNameJose C
person.identifier41146
person.identifierG-3003-2013
person.identifier.ciencia-id0115-071E-87E8
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.ciencia-id1713-6DA1-A551
person.identifier.orcid0000-0003-0772-8953
person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0002-4832-4341
person.identifier.ridJ-8697-2013
person.identifier.ridF-3014-2010
person.identifier.scopus-author-id36857887700
person.identifier.scopus-author-id10140393000
person.identifier.scopus-author-id7203033313
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication08f4685f-b917-410a-b153-59911ff7d75e
relation.isAuthorOfPublication.latestForDiscoverye10d78be-e547-4d25-92b5-06a997ed78da
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