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Evaluation of Rubus grandifolius L. (wild blackberries) activities targeting management of type-2 diabetes and obesity using in vitro models

2019Journal article Restricted access
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dc.contributor.authorSpínola, Vítor
dc.contributor.authorPinto, Joana
dc.contributor.authorLlorent-Martínez, Eulogio J.
dc.contributor.authorTomás, Helena
dc.contributor.authorCastilho, Paula C.
dc.date.accessioned2019-07-10T14:04:23Z
dc.date.available2019-07-10T14:04:23Z
dc.date.issued2019
dc.description.abstractRubus grandifolius Lowe (wild blackberries) is an endemic species from Madeira Archipelago (Portugal) used in folk medicine for alleviating diabetic complications. In this work, R. grandifolius methanolic extracts were analysed for in vitro inhibitory effect on digestive enzymes linked to type-2 diabetes, as well as aldose reductase activity and protein glycation. The phenolic composition, antioxidant and cytotoxic activities were also determined. Methanolic extracts exhibited strong inhibition of glucosidases (α- and β), but were less potent for α-amylase and pancreatic lipase when compared to current pharmaceutical drugs. The total phenolic content determined by HPLC-DAD varied between 92.96 - 97.47 and 118.01-137.41 mg g-1 of dry extract for berries and leaves, respectively. Fifty polyphenols were quantified, anthocyanins and ellagitannins being the main compounds. Cyanidin-3-glucoside was identified as one of the main hypoglycaemic and hypolipidemic agents in all extracts. R. grandifolius also prevented glycation of bovine-serum albumin (BSA) and showed strong radical scavenging activity against tested free radicals. At low concentration, the extracts were not cytotoxic against Caco-2 cells. Based on the results of this study, wild blackberry extracts demonstrated a potential beneficial effect on the control/management of type-2 diabetes mellitus, validating their use in folk medicine.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSpínola, V., Pinto, J., Llorent-Martínez, E. J., Tomás, H., & Castilho, P. C. (2019). Evaluation of Rubus grandifolius L.(wild blackberries) activities targeting management of type-2 diabetes and obesity using in vitro models. Food and Chemical Toxicology, 123, 443-452.pt_PT
dc.identifier.doi10.1016/j.fct.2018.11.006pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10400.13/2476
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.subjectRubus grandifolius L.pt_PT
dc.subjectPolyphenolspt_PT
dc.subjectDigestive enzyme inhibitionpt_PT
dc.subjectAldose reductasept_PT
dc.subjectAdvanced glycation end-productspt_PT
dc.subjectAntioxidant activitypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleEvaluation of Rubus grandifolius L. (wild blackberries) activities targeting management of type-2 diabetes and obesity using in vitro modelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F84672%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.citation.endPage452pt_PT
oaire.citation.startPage443pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume123pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSpínola
person.familyNameNunes Pinto
person.familyNameTomás
person.familyNameCastilho
person.givenNameVítor
person.givenNameJoana Carolina
person.givenNameHelena
person.givenNamePaula C
person.identifier556975
person.identifierA-4412-2013
person.identifier.ciencia-id0412-6D53-3A09
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.orcid0000-0003-2456-8613
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0002-8303-4286
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id54881258000
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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