A carregar...
Publicação
Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 873.2 KB | Adobe PDF |
Orientador(es)
Resumo(s)
The present study developed a two-step strategy to enhance the production of extracellular polymeric substances (EPSs) by a
thermotolerant chlorophyte, Graesiella sp., in view to their industrial valorisation. In the first step, Graesiella sp. was grown in
outdoor conditions in pilot-scale photobioreactors of 100 L culture volumes. In the second step, the biomass collected in the
exponential growth phase was submitted to heat stress (50 °C). A joint production of biomass reaching 0.50 gdw L−1 day−1 and of
EPS production reaching 1.30 gdw L−1 in 2 days was obtained. EPSs mainly contained polysaccharides (80%) and proteins
(14%). FTIR and 1HNMR revealed the presence of primary amine and sulfated groups. The EPSs contained antioxidant enzymes
(SOD, CAT, and APX) maintained in an active state by the microenvironment offered by the EPSs. The EPSs were found to have
a potent antioxidant activity via directly scavenging free radicals when compared to L-ascorbic acid.
Descrição
Palavras-chave
Microalgae Graesiella Chlorophyta EPSs Antioxidant enzymes Characterization Radical scavenging Outdoor cultivation Two-step strategy . Faculdade de Ciências Exatas e da Engenharia
Contexto Educativo
Citação
Gongi, W., Cordeiro, N., Pinchetti, J. L. G., Sadok, S., & Ouada, H. B. (2021). Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp. Journal of Applied Phycology, 33(1), 357-369.
Editora
Springer