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Evidences of organic acids exudation in aluminium stress responses of two Madeiran wheat (Triticum aestivum L.) landraces
dc.contributor.author | Rodrigues, Marta | |
dc.contributor.author | Ganança, José F.T. | |
dc.contributor.author | Silva, Emanuel M. da | |
dc.contributor.author | Santos, Teresa M. M. dos | |
dc.contributor.author | Slaski, Jan J. | |
dc.contributor.author | Zimny, Janusz | |
dc.contributor.author | Carvalho, Miguel Â. A. Pinheiro de | |
dc.date.accessioned | 2021-03-05T12:01:07Z | |
dc.date.available | 2021-03-05T12:01:07Z | |
dc.date.issued | 2019 | |
dc.description.abstract | Two wheat (Triticum aestivum L.) Madeiran landraces were subjected to 100 μM and 200 μM of aluminium (Al) in hydroponic culture, assessing the organic acid exudation role in plant’s responses to this metal. Samples of initial landrace populations (F0), F3 and haplodiploid lines (DH) were evaluated using standard tests: eriochrome cyanine R staining, root elongation and callose accumulation in roots. Root exudates were obtained to determine if the accumulation of malic and citric acids in hydroponic medium was a response to Al exposure. Additionally, the presence of ALMT1 gene was determined using five microsatellite markers. Standard tests confirmed that ISOP 76 was Al tolerant and ISOP 239, Al susceptible. ISOP 76, in the presence of 100 μM Al, exuded substantially more malic acid (12.87 to 43.33 mg/L), than ISOP 239 (3.65 to 7.72 mg/L). The levels of both organic acid exudation were substantially lower in ISOP 239 than in the ISOP 76. In the presence of 200 μM Al, ISOP 76 F0 shows a higher root elongation ratio (better tolerates Al), but the DH line was the one that exuded higher content of malic acid. Different gene alleles and promoters were detected in both landraces. Molecular differences could explain the observed dissimilarity in organic acid exudation response to Al stress. | pt_PT |
dc.description.sponsorship | The authors are grateful to the Madeiran farmers providing samples of wheat landraces, and to all staff of CGR ISOPlexis Gene bank and of the Department of Biological Sciences of Alberta University for support and access to laboratory facilities. This work got the funding support from the programs INTERREG III-B, contract 05/MAC/4.1/C15 and Madeira PO 14-20, contract CASBio, refª. M1420-01-0145-FEDER-000011. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.citation | Rodrigues, M., Ganança, J. F. T., Silva, E. M., dos Santos, T. M., Slaski, J. J., Zimny, J., & Carvalho, M. Â. P. (2019). Evidences of organic acids exudation in aluminium stress responses of two Madeiran wheat (Triticum aestivum L.) landraces. Genetic Resources and Crop Evolution, 66(4), 857-869. https://doi.org/10.1007/s10722-019-00754-0 | pt_PT |
dc.identifier.doi | 10.1007/s10722-019-00754-0 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10400.13/3153 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | Springer Nature | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.subject | Haplodiploid lines | pt_PT |
dc.subject | Aluminium tolerance | pt_PT |
dc.subject | Eriochrome cyanine R staining | pt_PT |
dc.subject | Organic acids exudation | pt_PT |
dc.subject | ALMT1 gene | pt_PT |
dc.subject | Microsatellite markers | pt_PT |
dc.subject | . | pt_PT |
dc.subject | Faculdade de Ciências da Vida | pt_PT |
dc.title | Evidences of organic acids exudation in aluminium stress responses of two Madeiran wheat (Triticum aestivum L.) landraces | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.citation.endPage | 869 | pt_PT |
oaire.citation.issue | 4 | pt_PT |
oaire.citation.startPage | 857 | pt_PT |
oaire.citation.title | Genetic Resources and Crop Evolution | pt_PT |
oaire.citation.volume | 66 | pt_PT |
person.familyName | Rodrigues | |
person.familyName | Ganança | |
person.familyName | da Silva | |
person.familyName | Almeida Pinheiro de Carvalho | |
person.givenName | Marta | |
person.givenName | José Filipe | |
person.givenName | Emanuel Marques | |
person.givenName | Miguel Angelo | |
person.identifier | 1218558 | |
person.identifier.ciencia-id | AE1E-00A2-3951 | |
person.identifier.ciencia-id | 4610-6741-6816 | |
person.identifier.orcid | 0000-0002-4783-9781 | |
person.identifier.orcid | 0000-0002-8384-6188 | |
person.identifier.orcid | 0000-0002-2505-1045 | |
person.identifier.orcid | 0000-0002-5084-870X | |
person.identifier.rid | AEE-0913-2022 | |
person.identifier.scopus-author-id | 8758577600 | |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |
relation.isAuthorOfPublication | e3352008-f600-47a1-ab18-403b1febd79b | |
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relation.isAuthorOfPublication | 2e433c48-88f8-40a2-b21f-de78b44aab1c | |
relation.isAuthorOfPublication.latestForDiscovery | 21d8a5a0-c756-4057-994e-6aba453ce764 |
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