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A fast and innovative microextraction technique, μSPEed, followed by ultrahigh performance liquid chromatography for the analysis of phenolic compounds in teas

2015Journal article Restricted access
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dc.contributor.authorPorto-Figueira, Priscilla
dc.contributor.authorFigueira, José A.
dc.contributor.authorPereira, Jorge A. M.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-22T10:07:50Z
dc.date.available2019-07-22T10:07:50Z
dc.date.issued2015
dc.description.abstractThe objective of this study was to evaluate the efficiency of a promising solid phase microextraction technique, μSPEed, in the analysis of selected phenolic compounds from teas by ultrahigh performance liquid chromatography with photodiode array detection (μSPEed/UHPLC-PDA). The innovative μSPEed configuration uses 3-μm sorbent particles tightly packed in a disposable needle equipped with a pressure-driven valve to withdraw samples in a single direction. The system was operated by the electronic pipette eVol® and different parameters influencing the extraction efficiency, as the nature of sorbent, pH, loading and elution conditions, and solvents were optimized. The best extracting conditions were obtained by loading twice 100μL of tea samples through the PS/DVB-RP sorbent and eluting with 50μL of acidified MeOH 95%. The following chromatographic separation was carried out in an Acquity C18 BEH capillary column using a gradient of 0.1% FA and acetonitrile. The optimized μSPEed/UHPLC-PDA methodology is selective and specific and was properly validated for 8 phenolic compounds widely reported in different teas. Overall, an excellent analytical performance was obtained in the 0.2-20μg/L linear dynamic range (LDR), with very low limits of detection (LODs) and quantification (LOQs), ranging between 3.5-16.8ng/mL and 10.6-50.6ng/mL, respectively, high recoveries (89.3-103.3%), good precision (RSD<5%) and negligible matrix effect. The methodology was used to assess the target polyphenols concentration in several tea samples. Rutin and quercetin-3-glucoside were the most abundant phenolics in all tea samples analysed and, with exception of naringenin and cinnamic acid, which are present in high amounts in the investigated citric teas, remain phenolic compounds are present in trace levels.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPorto-Figueira, P., Figueira, J. A., Pereira, J. A., & Câmara, J. S. (2015). A fast and innovative microextraction technique, μSPEed, followed by ultrahigh performance liquid chromatography for the analysis of phenolic compounds in teas. Journal of Chromatography A, 1424, 1-9.pt_PT
dc.identifier.doi10.1016/j.chroma.2015.10.063pt_PT
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10400.13/2492
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectμ-SPEedpt_PT
dc.subjectUHPLC-PDApt_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectTeaspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleA fast and innovative microextraction technique, μSPEed, followed by ultrahigh performance liquid chromatography for the analysis of phenolic compounds in teaspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66177%2F2009/PT
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Chromatography Apt_PT
oaire.citation.volume1424pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNamePorto-Figueira
person.familyNameOliveira Figueira
person.familyNameAugusto Machado Pereira
person.familyNameCâmara
person.givenNamePriscilla
person.givenNameJosé Aldónio
person.givenNameJorge
person.givenNameJosé
person.identifierA-8724-2017
person.identifierG-3003-2013
person.identifier.ciencia-idAD11-4860-FB1B
person.identifier.ciencia-id0B1E-B3DD-95F5
person.identifier.ciencia-idEC12-EE8F-50E8
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0001-7773-182X
person.identifier.orcid0000-0003-3719-9327
person.identifier.orcid0000-0003-0316-5348
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id56724173300
person.identifier.scopus-author-id57201813505
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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