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Exploring the potentialities of an improved ultrasound-assisted quick, easy, cheap, effective, rugged, and safe-based extraction technique combined with ultrahigh pressure liquid chromatography-fluorescence detection for determination of Zearalenone in cereals

2015Journal article Restricted access
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dc.contributor.authorPorto-Figueira, Priscilla
dc.contributor.authorCamacho, Irene
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-19T15:08:18Z
dc.date.available2019-07-19T15:08:18Z
dc.date.issued2015
dc.description.abstractZearalenone (ZEA), a secondary metabolite from Fusarium graminearum fungi that invade crops and grow during blooming in moist cool field conditions, is a nonsteroidal oestrogen, biologically very potent, although hardly toxic. Different analytical methods, among which a quick, easy, cheap, effective, rugged, and safe (QuEChERS) strategy, combined with or liquid chromatography, have been proposed for ZEA determination in foods. However, this extraction procedure has the disadvantage of using large amount of organic solvents, partitioning salts and sample volume, which limits the technique application on the fields where the sample amount is critical. Therefore in this work an improved sample preparation step based on the original QuEChERS (O-QuEChERS) approach, the μ-QuEChERS, combined with ultrasound-assisted extraction (USAE) and ultrahigh pressure liquid chromatography (UHPLC) analysis with fluorescence detection (FLR) is proposed for the sensitive and high-throughput quantification of ZEA in cereals. The proposed analytical strategy, μ-QuEChERSUSAE, uses up to 32 times lower amounts of partitioning salts, lower sample amount and lower extraction solvents, in comparison with O-QuEChERS technique. The performance of the analytical approach was assessed by studying the selectivity, specificity, limits of detection and quantification, linear dynamic range, matrix effect and precision. Good linearity (r(2)>0.99) was achieved for ZEA, and limits of detection (LOD=3.4μgkg(-1)) and limits of quantification (LOQ=4.7μgkg(-1)) were found below the tolerance levels set by European Commission. Good recoveries were obtained with different spiked concentrations, ranged from 80.2% to 109.7%, with relative standard deviations (RSD) lower than 5.0%. The comparison of the analytical performance of the proposed μ-QuEChERSUSAE with O-QuEChERS showed the powerful ability of the proposed strategy since it gives comparable results but using lower amounts of sample, partitioning salts and volume of the organic solvent, revealing of great potential and improvement of the proposed method.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPorto-Figueira, P., Camacho, I., & Câmara, J. S. (2015). Exploring the potentialities of an improved ultrasound-assisted quick, easy, cheap, effective, rugged, and safe-based extraction technique combined with ultrahigh pressure liquid chromatography-fluorescence detection for determination of Zearalenone in cereals. Journal of Chromatography A, 1408, 187-196.pt_PT
dc.identifier.doi10.1016/j.chroma.2015.07.031pt_PT
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10400.13/2491
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectCerealspt_PT
dc.subjectZearalenonept_PT
dc.subjectO-QuEChERSpt_PT
dc.subjectμ-QuEChERSpt_PT
dc.subjectUltrasound assisted extractionpt_PT
dc.subjectUltra high pressure liquid chromatographypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleExploring the potentialities of an improved ultrasound-assisted quick, easy, cheap, effective, rugged, and safe-based extraction technique combined with ultrahigh pressure liquid chromatography-fluorescence detection for determination of Zearalenone in cerealspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.citation.endPage196pt_PT
oaire.citation.startPage187pt_PT
oaire.citation.titleJournal of Chromatography Apt_PT
oaire.citation.volume1408pt_PT
oaire.fundingStream5876
person.familyNamePorto-Figueira
person.familyNameCamacho
person.familyNameCâmara
person.givenNamePriscilla
person.givenNameIrene
person.givenNameJosé
person.identifierG-3003-2013
person.identifier.ciencia-idAD11-4860-FB1B
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0001-7773-182X
person.identifier.orcid0000-0003-0061-905X
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id56724173300
person.identifier.scopus-author-id56374101100
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa617cf60-23e3-40ac-9ca8-f0d4b867d8e2
relation.isAuthorOfPublication5b2a49ac-c192-4d42-80f8-28efc578f53e
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscovery5b2a49ac-c192-4d42-80f8-28efc578f53e
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