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Alternative oxidase gene family in Hypericum perforatum L.: characterization and expression at the post-germinative phase

dc.contributor.authorVelada, Isabel
dc.contributor.authorCardoso, Hélia G.
dc.contributor.authorRagonezi, Carla
dc.contributor.authorNogales, Amaia
dc.contributor.authorFerreira, Alexandre
dc.contributor.authorValadas, Vera
dc.contributor.authorArnholdt-Schmitt, Birgit
dc.date.accessioned2022-02-07T10:36:47Z
dc.date.available2022-02-07T10:36:47Z
dc.date.issued2016
dc.description.abstractAlternative oxidase (AOX) protein is located in the inner mitochondrial membrane and is encoded in the nuclear genome being involved in plant response upon a diversity of environmental stresses and also in normal plant growth and development. Here we report the characterization of the AOX gene family of Hypericum perforatum L. Two AOX genes were identified, both with a structure of four exons (HpAOX1, acc. KU674355 and HpAOX2, acc. KU674356). High variability was found at the N-terminal region of the protein coincident with the high variability identified at the mitochondrial transit peptide. In silico analysis of regulatory elements located at intronic regions identified putative sequences coding for miRNA precursors and trace elements of a transposon. Simple sequence repeats were also identified. Additionally, the mRNA levels for the HpAOX1 and HpAOX2, along with the ones for the HpGAPA (glyceraldehyde-3-phosphate dehydrogenase A subunit) and the HpCAT1 (catalase 1), were evaluated during the post-germinative development. Gene expression analysis was performed by RT-qPCR with accurate data normalization, pointing out HpHYP1 (chamba phenolic oxidative coupling protein 1) and HpH2A (histone 2A) as the most suitable reference genes (RGs) according to GeNorm algorithm. The HpAOX2 transcript demonstrated larger stability during the process with a slight down-regulation in its expression. Contrarily, HpAOX1 and HpGAPA (the corresponding protein is homolog to the chloroplast isoform involved in the photosynthetic carbon assimilation in other plant species) transcripts showed a marked increase, with a similar expression pattern between them, during the post-germinative development. On the other hand, the HpCAT1 (the corresponding protein is homolog to the major H2O2-scavenging enzyme in other plant species) transcripts showed an opposite behavior with a down-regulation during the process. In summary, our findings, although preliminary, highlight the importance to investigate in more detail the participation of AOX genes during the post-germinative development in H. perforatum, in order to explore their functional role in optimizing photosynthesis and in the control of reactive oxygen species (ROS) levels during the process.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVelada, I., Cardoso, H. G., Ragonezi, C., Nogales, A., Ferreira, A., Valadas, V., & Arnholdt-Schmitt, B. (2016). Alternative oxidase gene family in Hypericum perforatum L.: characterization and expression at the post-germinative phase. Frontiers in Plant Science, 7, 1043.pt_PT
dc.identifier.doi10.3389/fpls.2016.01043pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4052
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCyanide-resistant pathwaypt_PT
dc.subjectSt. John’s Wortpt_PT
dc.subjectPlant developmentpt_PT
dc.subjectGene expressionpt_PT
dc.subjectTransposable elementspt_PT
dc.subjectmiRNAspt_PT
dc.subjectAlternative oxidasept_PT
dc.subjectPost-germinationpt_PT
dc.subject.pt_PT
dc.subjectEscola Superior de Tecnologias e Gestãopt_PT
dc.titleAlternative oxidase gene family in Hypericum perforatum L.: characterization and expression at the post-germinative phasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00115%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-GPL%2F099263%2F2008/PT
oaire.citation.titleFrontiers in Plant Sciencept_PT
oaire.citation.volume7pt_PT
oaire.fundingStream5876
oaire.fundingStream5876-PPCDTI
person.familyNameRagonezi
person.givenNameCarla
person.identifier.ciencia-idFC12-D3F0-EF3D
person.identifier.orcid0000-0002-1822-5473
person.identifier.scopus-author-id36460033800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa51cc988-63ce-40e5-9b71-a27b1eadc937
relation.isAuthorOfPublication.latestForDiscoverya51cc988-63ce-40e5-9b71-a27b1eadc937
relation.isProjectOfPublication521c2875-a47d-4804-930e-d2a21a2c489b
relation.isProjectOfPublication576b6af5-017d-4190-ad20-013bff9aca14
relation.isProjectOfPublication.latestForDiscovery576b6af5-017d-4190-ad20-013bff9aca14

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