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Gene delivery using biodegradable polyelectrolyte microcapsules prepared through the layer-by-layer technique

2012Journal article Restricted access
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dc.contributor.authorSantos, José L.
dc.contributor.authorNouri, Alireza
dc.contributor.authorFernandes, Tiago
dc.contributor.authorRodrigues, João
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-06-19T09:41:11Z
dc.date.available2019-06-19T09:41:11Z
dc.date.issued2012
dc.description.abstractBiodegradable and non-biodegradable microcapsules were prepared via the layer-by-layer (LbL) technique consisting of the polyelectrolyte pairs of dextran sulphate/poly-L-arginine and poly(styrene sulfonate)/poly(allylamine hydrochloride), respectively, in an attempt to encapsulate plasmid DNA (pDNA) for efficient transfection into NIH 3T3 cells. Results indicated the retention of bioactivity in the encased pDNA, as well as a correlation between the level of in vitro gene expression and biodegradability properties of polyelectrolyte. Furthermore, the incorporation of iron oxide nanoparticles within the polyelectrolyte layers significantly improved the in vitro transfection efficiency of the microcapsules. As a novel pDNA delivery system, the reported biodegradable microcapsules provide useful insight into plasmid-based vaccination and where there is a prerequisite to deliver genes into cells capable of phagocytosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSantos, J. L., Nouri, A., Fernandes, T., Rodrigues, J., & Tomás, H. (2012). Gene delivery using biodegradable polyelectrolyte microcapsules prepared through the layer‐by‐layer technique. Biotechnology progress, 28(4), 1088-1094.pt_PT
dc.identifier.doi10.1002/btpr.1576pt_PT
dc.identifier.issn8756-7938
dc.identifier.urihttp://hdl.handle.net/10400.13/2408
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Institute of Chemical Engineerspt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectBiodegradable polymerpt_PT
dc.subjectGene deliverypt_PT
dc.subjectLayer-by-layer techniquept_PT
dc.subjectMicrocapsulespt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleGene delivery using biodegradable polyelectrolyte microcapsules prepared through the layer-by-layer techniquept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage1094pt_PT
oaire.citation.startPage1088pt_PT
oaire.citation.titleBiotechnology progresspt_PT
oaire.citation.volume28(4)pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameNouri
person.familyNameRodrigues
person.familyNameTomás
person.givenNameAlireza
person.givenNameJoão
person.givenNameHelena
person.identifier556975
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0003-3532-1309
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridB-6816-2008
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb49d98f8-099f-4168-8732-340e5f50ff30
relation.isAuthorOfPublication53031fca-b2e7-4ce4-bda9-61e486da4547
relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
relation.isAuthorOfPublication.latestForDiscoveryb49d98f8-099f-4168-8732-340e5f50ff30
relation.isProjectOfPublication595c1582-33c0-493a-8ab3-37d2811536d9
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