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Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira

2019Journal article Open access
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dc.contributor.authorNunes, N.
dc.contributor.authorRosa, G. P.
dc.contributor.authorFerraz, S.
dc.contributor.authorBarreto, Maria Carmo
dc.contributor.authorCarvalho, M. A. A. Pinheiro de
dc.date.accessioned2021-03-08T11:36:09Z
dc.date.available2021-03-08T11:36:09Z
dc.date.issued2019
dc.description.abstractThree macroalgae collected at Madeira Island were included in this study to determine their potential for drug, nutraceutical, food, or supplement application. Fatty acid content was higher in Zonaria tournefortii (12.32 mg g−1 dw) with 16.58% of PUFAs, eicosapentaenoic acid (C20:5ω3), and arachidonic acid (20:4ω6) having concentrations of 2.59 and 1.17%, respectively. The anti-thrombogenic and anti-atherogenicity potential was higher for Z. tournefortii due to relevant fatty acids in the biochemical composition this macroalgae. Lipid classes were assessed in the lipid extract and neutral lipids (NL) were in higher yield in Asparagopsis taxiformis (51.16%) and lower in Z. tournefortii (26.96%). The glycolipids (GL) were between 36.03 and 16.11% in Z. tournefortii and Ulva lactuca. Phospholipids (PL) fraction varied from 35.91 and 31.60% in A. taxiformis and Z. tournefortii. TLC screening identified that U. lactuca contains phytol and cholesterol in its NL, digalactosyldiacylglycerol in its GL, and cardiolipin and L-α-phosphatidylcholine in its PL. Zonaria tournefortii contains phytol and cholesterol in its NL classes, and the PL classes contain L-α-phosphatidylethanolamine and 1-(3-sn-phosphatidyl)-rac-glycerol. The macroalgae A. taxiformis revealed cholesterol in its NL fraction and the same phospholipids as Z. tournefortii in its PL fraction. ATRFTIR analysis enabled a “fingerprint” spectra and important sulfation absorption bands were identified, revealing the functional polysaccharides within these macroalgae. Anti-cholinesterasic activity was assessed in A. taxiformis, with a low IC50 for AChE (8.92 ± 0.43 μg mL−1 ) and BuChE (13.96 ± 0.32 μg mL−1 ), demonstrating dual inhibitory activity, justifying the interest to identify the active principle which may be the scaffold of a novel drug.pt_PT
dc.description.sponsorshipThis work was financially supported by DemoBlueAlgae “Desenvolvimento de metodologias e optimização dos processos de cultivo e processamento de macroalgas para a indústria e economia azul” PROCiência 2020 (M1420-01-0247-FEDER000002);MACBIOBLUE “Proyecto demostrativo y de transferencia tecnológica para ayudar a las empresas a desarrollar nuevos produtos y procesos en el ambito de la Biotecnología Azul de la Macaronesia” (MAC/1.1b/ 086), program Interreg MAC 2014–2020; ARDITI - Regional Agency for the Development of Research Technology and Innovation (M14-20-09- 5369-FSE-000001-Doctorate in Business; Blue Iodine II “Boost Blue economy through market uptake an innovative seaweed bioextract for iodine fortification II”, grant agreement no. 733552, H2020-SMEInst2016-2017.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNunes, N., Rosa, G. P., Ferraz, S., Barreto, M. C., & Carvalho, M. P. (2019). Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira. Journal of Applied Phycology, 1-13. https://doi.org/10.1007/s10811-019-01884-9pt_PT
dc.identifier.doi10.1007/s10811-019-01884-9pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3156
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationCentre for Ecology, Evolution and Environmental Changes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSeaweedspt_PT
dc.subjectAsparagopsis taxiformispt_PT
dc.subjectUlva lactucapt_PT
dc.subjectZonaria tournefortiipt_PT
dc.subjectLipidspt_PT
dc.subjectPUFAspt_PT
dc.subjectMUFAspt_PT
dc.subjectSFAspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleFatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeirapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Ecology, Evolution and Environmental Changes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIA%2F00329%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIA%2F00329%2F2019/PT
oaire.citation.endPage771pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage759pt_PT
oaire.citation.titleJournal of Applied Phycologypt_PT
oaire.citation.volume32pt_PT
oaire.fundingStream5876
oaire.fundingStream6817 - DCRRNI ID
person.familyNameNunes
person.familyNameFerraz Alves
person.familyNameRoque Lino Felgueiras Barreto
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameNuno
person.givenNameSónia
person.givenNameMaria do Carmo
person.givenNameMiguel Angelo
person.identifierN-8704-2015
person.identifier1218558
person.identifier.ciencia-idD412-34DB-BB84
person.identifier.ciencia-id3716-94D4-BF42
person.identifier.ciencia-idC512-A6FD-4A68
person.identifier.ciencia-id4610-6741-6816
person.identifier.orcid0000-0001-6166-8045
person.identifier.orcid0000-0001-7939-1371
person.identifier.orcid0000-0001-5797-8913
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridAEE-0913-2022
person.identifier.scopus-author-id7004455600
person.identifier.scopus-author-id8758577600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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