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Characterization of volatile substances in apples from rosaceae family by headspace solid‐phase microextraction followed by GC‐qMS

2009-03Journal article Open access
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dc.contributor.authorFerreira, Liseth
dc.contributor.authorPerestrelo, Rosa Maria de Sá
dc.contributor.authorCaldeira, Michael Manuel Lima
dc.contributor.authorCâmara, José S.
dc.date.accessioned2015-12-11T14:29:58Z
dc.date.available2015-12-11T14:29:58Z
dc.date.issued2009-03
dc.description.abstractThe volatile composition of different apple varieties of Malus domestica Borkh. species from different geographic regions at Madeira Islands, namely Ponta do Pargo (PP), Porto Santo (PS), and Santo da Serra (SS) was established by headspace solid-phase microextraction (HS-SPME) procedure followed by GC-MS (GC-qMS) analysis. Significant parameters affecting sorption process such as fiber coating, extraction temperature,extractiontime,sampleamount,dilutionfactor,ionicstrength,anddesorption time,wereoptimizedanddiscussed.TheSPMEfibercoatedwith50/30 lmdivinylbenzene/carboxen/PDMS (DVB/CAR/PDMS) afforded highest extraction efficiency of volatile compounds, providing the best sensitivity for the target volatiles, particularly whenthesampleswereextractedat508Cfor30 minwithconstantmagneticstirring. A qualitative and semi-quantitative analysis between the investigated apple species has been established. It was possible to identify about 100 of volatile compounds amongpulp(46,45,and39),peel(64,60,and64),andentirefruit(65,43,and50)inPP, PS,andSSapples,respectively.Ethylesters,terpenes,andhigheralcoholswerefound tobethemostrepresentativevolatiles. a-Farnesene,hexan-1-olandhexyl2-methylbutyratewerethecompoundsfoundinthevolatileprofileofstudiedappleswiththelargestGCarea,representing,onaverage,24.71,14.06,and10.80%ofthetotalvolatilefractionfromPP,PS,andSSapples.InPPentireapple,themostabundantcompoundsidentified were a-farnesene (30.49%), the unknown compound m/z (69, 101, 157) (21.82%) andhexylacetate(6.57%).RegardingPSentireapplethemajorcompoundswere a-farnesene(16.87%),estragole(15.43%),hexan-1-ol(10.94),andE-2-hexenal(10.67).a-Farnesene(30.3%),hexan-1-ol(18.90%),2-methylbutanoicacid(4.7%),andpentan-1-ol(4.6%) werealsofoundasSSentireapplevolatilespresentinahigherrelativecontent.Principal component analysis (PCA) of the results clustered the apples into three groups according to geographic origin. Linear discriminant analysis (LDA) was performed in order to detect the volatile compounds able to differentiate the three kinds of apples investigated. The most important contributions to the differentiation of the PP, PS, and SS apples were ethyl hexanoate, hexyl 2-methylbutyrate, E,E-2,4-heptadienal, pethylstyrene,andE-2-hexenal.pt_PT
dc.identifier.citationFerreira, L., Perestrelo, R., Caldeira, M., & Câmara, J. S. (2009). Characterization of volatile substances in apples from rosaceae family by headspace solid‐phase microextraction followed by GC‐qMS. Journal of separation science, 32(11), 1875-1888.pt_PT
dc.identifier.doi10.1002/jssc.200900024pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/954
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWILEY-VCH Verlag GmbH & Co. KGaApt_PT
dc.subjectApplespt_PT
dc.subjectGC-qMSpt_PT
dc.subjectMalus domestica Borkh.pt_PT
dc.subjectSolid-phase microextractionpt_PT
dc.subjectVolatile profilept_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleCharacterization of volatile substances in apples from rosaceae family by headspace solid‐phase microextraction followed by GC‐qMSpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1888pt_PT
oaire.citation.startPage1875pt_PT
oaire.citation.titleJournal of separation sciencept_PT
oaire.citation.volume32(11)pt_PT
person.familyNamePerestrelo
person.familyNameCaldeira
person.familyNameCâmara
person.givenNameRosa
person.givenNameMichael
person.givenNameJosé
person.identifier1441319
person.identifierG-3003-2013
person.identifier.ciencia-id251A-D5F7-9E32
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-7223-1022
person.identifier.orcid0000-0001-5371-7281
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridI-2307-2014
person.identifier.ridA-2461-2013
person.identifier.scopus-author-id16686828800
person.identifier.scopus-author-id16300962200
person.identifier.scopus-author-id10140393000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication003ff241-47d2-497b-b9ac-c0d1713f8bf3
relation.isAuthorOfPublicationce73ce9c-932a-404b-93fe-cfefd5181554
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscovery003ff241-47d2-497b-b9ac-c0d1713f8bf3

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