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An attractive, sensitive and high-throughput strategy based on microextraction by packed sorbent followed by UHPLC-PDA analysis for quantification of hydroxybenzoic and hydroxycinnamic acids in wines

2013Journal article Restricted access
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dc.contributor.authorGonçalves, João
dc.contributor.authorSilva, Catarina L.
dc.contributor.authorCastilho, Paula C.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-18T13:51:54Z
dc.date.available2019-07-18T13:51:54Z
dc.date.issued2013
dc.description.abstractAn attractive, fast, low-consumption and very promising high-throughput strategy based on microextraction by packed sorbent (MEPS), and reversed phase ultra-high pressure liquid chromatography (UHPLC), was developed and validated for the determination and quantification of hydroxybenzoic (gallic acid, protocatechuic acid, gentisic acid, vanillic acid, syringic acid), and hydroxycinnamic acids (p-coumaric acid, m-coumaric acid, o-coumaric acid, ferrulic acid and cinnamic acid) in wines. Important factors affecting the performance of MEPS such as the type of sorbent material (C2, C8, C18, SIL and C8/SCX), number of extraction cycles (extract–discard), sample volume and elution volume were tested and optimized. The optimal MEPS extraction conditions were obtained using C8 phase as sorbent and 50 μL of the sample in five extraction cycles. The analytes were separated on a new Trifunctional High Strength Silica analytical column (HSS T3; 100% silica particle), specially designed for polar compounds, using a binary mobile phase composed of aqueous 0.1% formic acid (eluent A) and methanol (eluent B) in the gradient elution mode (10 min of total analysis). This new stationary phase, proved to be an excellent alternative to conventional C18 columns for the determination of benzoic and cinnamic acids derivatives in wines. The analytical method was fully validated in terms of linearity, limits of detection (LOD), limits of quantification (LOQ), extraction yield, accuracy and inter/intra-day precision, using a synthetic wine spiked with hydroxybenzoic and hydroxycinnamic acids, and demonstrated good linearity with r2‐values > 0.998 within the established concentration range (0.2–40 μg mL− 1). Under the optimized conditions, LOD of 0.01–0.2 μg mL− 1, LOQ of 0.03–0.7 μg mL− 1, and precision, expressed as the relative standard deviation (RSD %), within 0.3–6% were observed. The proposed method was also applied to the analysis of hydroxybenzoic and hydroxycinnamic acids in red and white wines and recoveries of 80–100% and 77–99%, respectively, were obtained. The new approach offers an attractive alternative for analysis of phenolic acids from wine samples, providing a number of advantages including decreased sample preparation, faster sample throughput and easy to perform as compared to traditional methodologies. Moreover it could potentially be extended to other extraction media.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGonçalves, J., Silva, C. L., Castilho, P. C., & Câmara, J. S. (2013). An attractive, sensitive and high-throughput strategy based on microextraction by packed sorbent followed by UHPLC-PDA analysis for quantification of hydroxybenzoic and hydroxycinnamic acids in wines. Microchemical Journal, 106, 129-138.pt_PT
dc.identifier.doi10.1016/j.microc.2012.05.037pt_PT
dc.identifier.issn0026-265X
dc.identifier.urihttp://hdl.handle.net/10400.13/2481
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectHydroxybenzoic acidspt_PT
dc.subjectHydroxycinnamic acidspt_PT
dc.subjectWinept_PT
dc.subjectMicroextraction by packed sorbentpt_PT
dc.subjectUltra high pressure liquid chromatographypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleAn attractive, sensitive and high-throughput strategy based on microextraction by packed sorbent followed by UHPLC-PDA analysis for quantification of hydroxybenzoic and hydroxycinnamic acids in winespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage138pt_PT
oaire.citation.startPage129pt_PT
oaire.citation.titleMicrochemical Journalpt_PT
oaire.citation.volume106pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGonçalves
person.familyNameSousa Luís
person.familyNameCastilho
person.familyNameCâmara
person.givenNameJoão
person.givenNameCatarina Grace
person.givenNamePaula C
person.givenNameJosé
person.identifierC-1300-2019
person.identifierA-4412-2013
person.identifierG-3003-2013
person.identifier.ciencia-id8C14-3188-9E44
person.identifier.ciencia-id9813-3B88-BA8D
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-1265-6686
person.identifier.orcid0000-0002-3018-3165
person.identifier.orcid0000-0002-8303-4286
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridC-1348-2019
person.identifier.scopus-author-id52163735200
person.identifier.scopus-author-id57194492726
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7971c475-3f08-4462-96b1-79e96c9615a4
relation.isAuthorOfPublication2853e6b6-0fb5-454c-aedb-05528b76c484
relation.isAuthorOfPublicationd84fd917-438e-43bf-ac6f-256562481c05
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