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Allergic asthma exhaled breath metabolome: a challenge for comprehensive two-dimensional gas chromatography

2012-09Journal article Open access
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dc.contributor.authorCaldeira, M.
dc.contributor.authorPerestrelo, Rosa Maria de Sá
dc.contributor.authorBarros, António S.
dc.contributor.authorBilelo, M. J.
dc.contributor.authorMorête, A.
dc.contributor.authorCâmara, J. S.
dc.contributor.authorRocha, Sílvia M.
dc.date.accessioned2015-12-17T09:25:42Z
dc.date.available2015-12-17T09:25:42Z
dc.date.issued2012-09
dc.description.abstractAllergic asthma represents an important public health issue, most common in the paediatric population, characterized by airway inflammation that may lead to changes in volatiles secreted via the lungs. Thus, exhaled breath has potential to be a matrix with relevant metabolomic information to characterize this disease. Progress in biochemistry, health sciences and related areas depends on instrumental advances, and a high throughput and sensitive equipment such as comprehensive two-dimensional gas chromatography–time of flight mass spectrometry (GC × GC–ToFMS) was considered. GC × GC–ToFMS application in the analysis of the exhaled breath of 32 children with allergic asthma, from which 10 had also allergic rhinitis, and 27 control children allowed the identification of several hundreds of compounds belonging to different chemical families. Multivariate analysis, using Partial Least Squares-Discriminant Analysis in tandem with Monte Carlo Cross Validation was performed to assess the predictive power and to help the interpretation of recovered compounds possibly linked to oxidative stress, inflammation processes or other cellular processes that may characterize asthma. The results suggest that the model is robust, considering the high classification rate, sensitivity, and specificity. A pattern of six compounds belonging to the alkanes characterized the asthmatic population: nonane, 2,2,4,6,6-pentamethylheptane, decane, 3,6-dimethyldecane, dodecane, and tetradecane. To explore future clinical applications, and considering the future role of molecular-based methodologies, a compound set was established to rapid access of information from exhaled breath, reducing the time of data processing, and thus, becoming more expedite method for the clinical purposes.pt_PT
dc.identifier.citationCaldeira, M., Perestrelo, R., Barros, A. S., Bilelo, M. J., Morete, A., Camara, J. S., & Rocha, S. M. (2012). Allergic asthma exhaled breath metabolome: a challenge for comprehensive two-dimensional gas chromatography. Journal of Chromatography A, 1254, 87-97.pt_PT
dc.identifier.doi10.1016/j.chroma.2012.07.023pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/962
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationDESENVOLV. DE UMA METODOLOGIA PARA A DETECÇÃO DE BIOMARCADORES DE DOENÇAS RESPIRATÓRIAS ALÉRGICAS BASEADA NA APLICAÇÃO DA MICROEXTRACÇÃO EM FASE SÓLIDA ACOPLADA A CROMATOGRAFIA EM FASE GASOSA. CONTRIBUTOS PARA A DEFINIÇÃO DE UM MEIO DIAGNÓSTICO
dc.subjectAllergic asthmapt_PT
dc.subjectExhaled breathpt_PT
dc.subjectVolatile metabolitespt_PT
dc.subjectHeadspace solid-phase microextractionpt_PT
dc.subjectComprehensive two-dimensional gas chromatography–time of flight mass spectrometrypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleAllergic asthma exhaled breath metabolome: a challenge for comprehensive two-dimensional gas chromatographypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDESENVOLV. DE UMA METODOLOGIA PARA A DETECÇÃO DE BIOMARCADORES DE DOENÇAS RESPIRATÓRIAS ALÉRGICAS BASEADA NA APLICAÇÃO DA MICROEXTRACÇÃO EM FASE SÓLIDA ACOPLADA A CROMATOGRAFIA EM FASE GASOSA. CONTRIBUTOS PARA A DEFINIÇÃO DE UM MEIO DIAGNÓSTICO
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PIDDAC/SFRH%2FBD%2F40374%2F2007/PT
oaire.citation.endPage97pt_PT
oaire.citation.startPage87pt_PT
oaire.citation.titleJournal of Chromatography Apt_PT
oaire.citation.volume1254pt_PT
oaire.fundingStreamPIDDAC
person.familyNameCaldeira
person.familyNamePerestrelo
person.familyNameBarros
person.familyNameCâmara
person.familyNameRocha
person.givenNameMichael
person.givenNameRosa
person.givenNameAntónio
person.givenNameJosé
person.givenNameSílvia M.
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person.identifier.orcid0000-0001-5371-7281
person.identifier.orcid0000-0002-7223-1022
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person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0002-0396-3019
person.identifier.ridA-2461-2013
person.identifier.ridI-2307-2014
person.identifier.scopus-author-id16300962200
person.identifier.scopus-author-id16686828800
person.identifier.scopus-author-id56130256800
person.identifier.scopus-author-id10140393000
person.identifier.scopus-author-id7006653213
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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